12 Wisconsin Academy of Sciences, Arts, and Letters. 
ness had occurred. In the remaining three trees traces of starch 
were present which soon disappeared. The radial growth 
ranged from 25% to normal. The tree defoliated June 15 was 
dead by October and the one defoliated in August by the follow¬ 
ing May. 
The stems of the first four and of some untreated young pines 
were cut in 15 to 30 cm. pieces in October and by December the 
bark on the treated-tree pieces was found to have loosened espe¬ 
cially where considerable radial growth had occurred. The bark 
had split and was shrunken both in length and circumference; 
while that on the pieces from untreated trees adhered firmly to 
the wood. In December pieces were also cut from the branches 
of the last treated tree, the bark of which had lost its turgidity 
after the operation but regained it again. A discolored circle 
was found in the cambial region. Groups of undifferentiated 
wood cells had been ruptured or broken down and were discol¬ 
ored. 
In his researches on the reserve food of trees du Sablon 26 
found that the carbohydrate content underwent farily definite 
seasonal changes which apparently occurred irrespective of the 
weather. On March 17 the roots of pear trees contained much 
more sugar and very much more starch than the stems and the 
total carbohydrate content of roots was also higher. In stems 
of chestnut trees the carbohydrate content reached a maximum 
in October and a minimum in May, while in roots the maximum 
came in September and the minimum in May. In case of quince 
the maximum in both root and stem was found in January with 
a minimum in stems in May and in roots in June. In peach the 
minimum in both root and stem came in May and the maximum 
in the stem in July and in roots in November. In willow both 
stem and roots were found to have a minimum of carbohydrates 
in April and a maximum in October, but both the maximum and 
minimum were more extreme in the roots. In the case of rasp¬ 
berry bushes the roots had a minimum in April and a maximum 
in October, while in the biennial stems a high carbohydrate con¬ 
tent was maintained during the first summer with a maximum in 
October, followed by a slight depression and subsequently a les¬ 
ser maximum in the second April. Afterwards a fairly constant 
20 du Sablon, Leclerc. Recherches physiologiques sur les matieres de 
reserves des arbres. Rev. Gen. Bot. 16:339-68; 386-401. 1904. 
