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BACTERIOLOGY. 
Diagnosis 
The disease may be transmitted to human beings 
from infected horses or may pass from man to man. 
The manifestations of the disease in man are much the 
same as in the horse. It may assume an acute or 
chronic course, the former nearly always resulting 
fatally. 
The toxins of the Bacillus mallei are within the 
bodies of the organisms, that is, they are endotoxins 
and are very resistant to heat. Attempts have been 
made to immunize animals by the injection of small 
amounts of the toxin, and have been to some extent 
successful. It is not possible to immunize man in this 
way. 
The diagnosis of glanders may be made in sev¬ 
eral ways. The discharges or the pus may be injected 
into the peritoneal cavity of guinea-pigs. If the 
bacillus of glanders is present the testicles become 
swollen and painful in two to five days. A test may 
be made for the presence of substances in the blood- 
serum that will agglutinate the bacilli of glanders. It 
is done in the same manner as the Widal reaction for 
typhoid fever. Finally, the toxin of the bacilli made 
from cultures and called mallein may be injected under 
the skin of suspected cases. If glanders is present it 
produces a reaction marked by fever and tenderness 
about the point of inoculation. The principle upon 
which this reaction rests is the same as in the tuber¬ 
culin reaction. 
