( 61 ) 
• of ammonium carbonate, for a culture obtained in this way had a 
titre of 0,18 N. The urea flora did not however directly follow that 
of B. fluorescens and allied species, for when a rough culture is 
only some days old it evolves a pleasant odour of a compound ester, 
and cultures show that the original species are replaced by an aroma 
producing'microbe. Milk used as medium for this bacterium favours 
very much the production of the aroma which after some days 
reminds of the flavour of young cheese. This bacterium, B. odoratum, 
does not however produce urease, although it is capable of splitting 
urea by catabolism. 
- To obtain the urea flora which succeeds that of B. odoratum, the 
culture was streaked on broth gelatin with addition of 1% urea and 
0,1 % ammonium carbonate. But no ureabacteria developed, which 
did take place on yeastwater gelatin with the above additions. A 
description of the thus isolated single species was not found; it shall 
be called Vrobacillus Musculi. The temperature of 30° is the most 
favourable for its culture as, especially on broth-agar urea and am¬ 
monium corbonate, then an abundance of urease-producing colonies 
occur. Sporulation has not been observed and gelatin is not liquefied. 
The maximum quantity of urea converted into a 1% peptone solution 
amounts to 3,6%* *). 
Addition of peptone is not quite necessary for the splitting of 
urea, but it highly favours it a ). Calcium malate, calcium lactate and 
glucose, together with urea, gave only rise to a very slight decom¬ 
position. 
3. Denitrification with uric acid as carbon food. 
If a stoppered bottle, quite filled with water and uric acid, with 
addition of 1 % kaliumnitrate and a little phosphate, is inoculated 
with soil, then after some days’ cultivation at 30° under these anae¬ 
robic conditions, gas evolves, which proves to consist of a mixture 
of carbonic acid and nitrogen. 
A plate culture on broth gelatin shows that the denitrifying bac¬ 
terium, B. Stutzeri B ), is cause of the process, hence this species may 
be very well accumulated in the said way. It also denitrifies strongly 
with uric acid in pure culture. In three days, for instance, 250 mG. 
KNO, were completely decomposed in 250 cM s . of the culture 
medium, so that sulphuric acid and diphenylamin did no more react. 
At denitrification in general the escaping nitrogen originates from the 
J ) Beijerinck, Centr.bl. f. Bacteriol. II, VII Bd. 1901. 
*) Sohmgen, Kon. Akad. v. Wetensch 12 N 1908. 
3 ) van Iterson, Central!)!, f. Bakteriologie II, XII Bd. 1902. 
