( 555 ) 
tnanee of muscular work leaves creatine unaffected”. Brown and 
Cathcart stimulated the muscles by means of the nerve and found 
a somewhat more considerable increase, of 7% k 13%, in four 
experiments, at which the circulation of the blood had been excluded. 
If the circulation was intact, they found a little diminution, not only 
with frogs, but also with rabbits, where it ought, however, to be 
taken into consideration that, in consequence of the stimulus, the 
muscle was more amply provided with blood, so that an exact 
comparison with resting muscles is scarcely possible. 
We have made experiments with frogs, in three different ways, 
always excluding the current of blood. First, after destroying brain 
and spinal cord and after section of the heart, the nervus ischiadicus 
on one side, laid bare high in the thigh, was cut through and stimu¬ 
lated with a series of rapidly succeeding induction-strokes. Each stimu¬ 
lation lasted V 2 minute, after which V, minute of rest was afforded 
during about one hour. In the second place the experiment was fofc 
the rest made in the same way, but the nerve was, for about half 
an hour, with the help of Engelmann’s rhythmic polyrheotome, stimu¬ 
lated 24 times per minute, alternately with a closing and an opening 
induction-stroke. At last two more experiments were made thus: the 
frog was cut through transversely in the lumbar region, after which 
the skin of the hind part was taken away. Now this was put astride 
on the partition of two basins of celluloid, standing against each other 
and filled with Ringer’s solution, so that each leg was immersed into 
the liquid to about half way up the thigh. Then the ischiadicus was 
on one side, from the pelvis, stimulated for half an hour, 24 times 
a minute, with single closing and opening induction-strokes. 
The quantity of creatine, expressed in mgr. creatinine per 1 grm. 
of muscle, was found as follows: 
Stimulated Rest Difference 
T 3 490 3 418 -4-0.072 
Stimulated Rest Difference 
[ I d.obb o.ooo — v.vdu 
) II 3.616 3.683 —0.067 
f III 3.796 3.856 — 0.060 
3.386 — 0.020 
A 
11 3.203 
^ IT 3 585 
It 3.585 
3.230 —0.027 
3.593 — 0.008 
