( 560 ) 
the muscle brought in contact with coffeine or with rhodane, whilst 
it continued reacting well upon the excitation, became gradually not 
inconsiderably shorter. 
The influence upon the quantity of creatine was examined in the 
usual way. The following figures were found: 
Ringers’ sol. - NaCNS 0.614 °/ 0 
I 2.822 3.098 
II 3.106 3.354 
III 3,051 3.537 
IV 3.129 3.459 
V 2.916 3.146 
Difference 
0.276 
0.330 
0.230 
Towards the end of the experiment the muscles did not contract 
any more. Stiffening was not to be perceived. 
Ringer’s liq. '' Citr. Coff. 
I 3.017 3.432 
II 3.055 3.623 
III 3.090 3.422 
IV 3.194 3.551 
V 3.316 3.519 
1:100 
1 :200 
1:400 
1:400 
1:800 
Difference 
0.415 
0.568 
0.332 
0.357 
0.203 
The leg brought in contact with coffeine was in I quite stiff after 
10 minutes, in II after a quarter of an hour, the contractions leaving 
off. In III, IV and V the contractions remained visible till the end 
of the experiment. Also in those cases the rigour was clear, though 
in V not so strongly as in III and IV. 
In all cases, without any exception, therefore, the quantity of 
creatine was found to be increased in the muscles that had been in 
tonus. The difference, except with coffeine, may even be estimated 
somewhat higher than the figures given, because, leaving the men¬ 
tioned exception out of consideration, the tonus appeared to be 
accompanied by a slight increase of the quantity of water. This 
difference is, however, so insignificant that it need not be taken into 
consideration. 
Increase of the quantity of creatine was found only then when 
the muscles had been brought into tonus by excitation. Immersion 
of the legs, during half an hour, in the solutions, without excitation, 
had no influence upon the quantity of creatine. The following expe¬ 
riments were made in the usual way, only with this difference, that 
the nerves were not excited. 
