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deep blue-violet, and this was also the ease with the half bordered 
pits between medullary raj 7 cells and fibres. The medullary rays and 
the xylem parenchyma were quite unstained, both as regards wall 
and contents. 
Experiment V. 
Larix decidua . 
A living leafy twig, 6 mm. thick at its base, stood for 5 days 
in the solution, after which the stain had penetrated to the apex. 
Staining completely limited to the wood, but no stain in the oldest 
of the 6 annual rings. 
The stain only taken up by a very thin layer of the wall, 
adjoining the 1 lumen of the tracheids and the cavities of the pits. 
Toms of the pits deep blue-violet, also in the half bordered pits 
between medullary rays and tracheids. For the rest everything 
unstained. 
Experiment VI. 
Salix spec. 
Two living leafless branches, provided at either end with a cut 
surface, both 30 cm. long and more than 1 / t cm. thick, stood for 
2 days in the aqueous solution of Saureviolett; one of the branches 
had its lower end in the solution, the other its upper end. 
The stain ascended readily, and in the two branches simultaneously. 
The stain only present in a thin layer of the wall adjoining the 
lumen of the vessels and fibres and the cavities of the pits. Tori 
deep blue-violet. 
Experiment VII. 
Fagus silvatica. Taxus baccata. 
Of each of these plants two similar 3—5 year old leafless branches 
were placed with the cut surface in the aqueous Saureviolett solution 
for 3 days. One of the branches of each species was alive, the other 
had been treated as follows. It had stood for L 1 /., hours in boiling 
water. Then water was sucked through the boiled branch by means 
of a filter pump in order to remove possible obstructions, finally a 
fresh surface was cut. 
39 * 
