{ 582 ) 
After 3 days the stajn Had almost reached the top in all the four 
branches. . 
In the living branches staining was scarcely visible against the 
walls of the vessels and tracheids. The tori, including those of the 
half bordered pits were deeply stained. Medullary ray- und parenchyma 
cells quite colourless. 
In the boiled branch of Fagus the walls of the libnform fibres 
and of the vessels were a uniform pale blue. Against the walls of 
the vessels in the spring wood a darker layer. Nowhere however 
coloured tori. The medullary rays also proved to be colourless. 
In the boiled branch of Taxus the walls of many tracheids w<?re 
stained a uniform pale blue; towards the inside against the walls a 
darker layer. The tori unstained. The medullary rays dark blue. 
Experiment VIII. 
Taxus baccata. 
Two living branches were taken. One was placed with its cut 
surface in a solution of 0.1 gram of Saureviolett in 100 c.c. of water ; 
the other in a solution of 0.1 gram of Saureviolett in 100 c.c, of alcohol 
Both branches remained standing in the solution for 43 hours, 
after which time sections were made through both at a height of 
7 cm. The staining was as follows: 
Branch in aqueous solution : staining only in the secondary xylein. 
A very thin blue layer against the walls of the tracheids, and of 
the cavities of the bordered pits. Tori dark blue, including those of 
the half bordered pits. Medullary rays unstained. 
Branch i n alcoholic solution-, the stain had also penetrated into 
the cambium and the innermost layers of the cortex parenchyma, 
where both walls and contents were dark blue. In the secondai) 
xylem the tracheid walls light blue; against the walls also clearly 
a blue layer, further in the cavities of the pits. Tori unstained. 
Medullary rays dark blue, both as regards walls and contents. 
The walls also coloured in the primary xylem. 
Experiment IX. 
Taxus baccata. 
A living branch was placed with the cut surface in a solution o* 
0.1 gram of Saureviolett in 100 e.c. of a 4 B /„ fommldehycle solution 
(diluted formalin). 
