( 70S ) 
in process of division and that on transferring them to distilled water 
or to ditch water the precipitate also disappeared somewhat sooner. 
For the sake of completeness I further mention, that no difference 
could be traced between the tannin content of cells in which the 
nuclear and cell division had just started, and the tannin content of 
cells not undergoing division, but the tannin content was found to 
have diminished, when the process of nuclear and cellular division 
was at its height or could be considered at an end. 
These results show, that a connexion must be looked for between 
the diminution of tannin content and the process of nuclear and 
cellular division. This process really consists of two processes, going 
on simultaneously, and therefore the question arose, which of* the 
two exerted its influence on the tannin content. With reference to 
this question I carried out some experiments. 
As has already been stated, the growth of the cells and the 
division of cell and nucleus is stopped in a one percent antipyrine 
solution or in a 0.1 percent caffeine solution. I therefore studied the 
effect of these solutions on the formation of transverse walls and on 
karyokinesis, when the dividing cells and those showing the very 
earliest signs of the process of nuclear and of cell division, were 
placed in these solutions for some time. Filaments, in which such 
cells occurred, were left for lh hours in the above mentioned solutions, 
and were then examined next day with regard to the division of 
cell and nucleus. The transverse walls, in process of formation, had 
been disturbed in their development, and therefore in these cases 
the cell was incompletely divided. The result in the cells which were 
on the point of dividing, when placed in the antipyrine- or caffeine 
solution, was more interesting; often in these cells no trace of a 
transverse wall could be found next day. The process of cell division 
had been completely suppressed. 
The process of nuclear division was however quite different. In 
all the cells where it was going on, or where it was about to begin, 
it had continued to the end and two normal daughter nuclei always 
resulted, which were generally situated a little apart in the axis ot 
the cell. 
It follows from these experiments, that a temporary fixation of 
the tannin by antipyrine or caffeine prevents the formation of trans¬ 
verse walls, but does not directly affect nuclear division. On the 
strength of this result I feel justified in assuming that there must 
be a connexion between the diminution of the tannin content, referred 
to above, and the formation of transverse walls. Both abolition of 
transverse wall formation through fixation of tannin and the dimi- 
