1174 Wisconsin Academy of Sciences, Arts, and Letters . 
represents a longitudinal section through the ascus at this stage. 
The spindle runs parallel with the long axis of the ascus and 
is very near the plasma membrane. The chromosomes are 
rather small and scattered along the spindle. The number of 
the chromosomes appears to be eight. The spindle is long and 
narrow, the fibres being attached at the poles to broad central 
bodies so that the poles of the spindle are very broad in compari¬ 
son with its diameter. The astral rays are strongly developed 
in this stage. The portions of the fibres near the central body 
stain almost as deeply as the central body itself does so that the 
latter is not easily distinguished from the fibres. 
Figure 2 represents the same stage as figure 1. Here the 
ascus has been cut somewhat obliquely through the side contain¬ 
ing the spindle and parallel with the spindle which runs at an 
oblique angle to the long axis. The spindle is not so near the 
plasma membrane as it is in figure 1. The cytoplasm is con¬ 
spicuously more dense in the vicinity of the spindle and becomes 
more vacuolate as the distance from the spindle increases. The 
spindle and astral rays appear very similar to those described for 
figure 1, being very distinct in both cases. The chromosome 
number again appears to he eight. In the cytoplasm are two 
large red-staining bodies, one to the right, the other above the 
spindle. The former appears as if it might be the remnant of 
the nucleole. 
At the close of this division the fibres disappear and the 
daughter nuclei round up. These nuclei are much smaller than 
the primary nucleus. When in the resting condition, they are 
to be found near the lower edge of the mass of spore plasm 
which now fills entirely the tip of the ascus. This spore plasm 
now shows a finely reticulated structure. Below the nuclei 
there appear to be a few rays running into the cytoplasm, but 
this condition is probably due to an irregular streaming of the 
cytoplasm since these rays are not oriented on a centrosome as 
are the kinoplasmic fibres of the division figures and since 
furthermore the rays often pass into the strands of cytoplasm 
which run down into the lower portions of the ascus. 
The spindles in the second division are similar to those of 
