Jolivette—Spore Formation. 
1175 
the first with the exception that the central bodies appear to be 
more flattened. The asters are also well developed here. They 
disappear at the close of the anaphase stage. When the four 
daughter nuclei are completely reconstructed, they are to be 
found near the lower part of the dense mass of spore plasm oc¬ 
cupying the upper portion of the ascus. They are so arranged 
that in longitudinal sections of the ascus only two can be seen in 
one plane. As you focus down below these two, the other two 
come into view so that a cross section through the ascus in the 
region of the nuclei shows all four in one plane. Of course 
in some cases their position may vary more or less. The spore 
plasm here as during the second division is finely reticulated. 
Figures 3 and 4 show the equatorial plate stage of the third 
division and were drawn from one section of an ascus but in 
different planes. The spindle is narrow with a broad distinct 
center at each end. The polar asters are extraordinarily well 
developed. The fibres are very long and distinct and they run 
out into the cytoplasm in all directions, some of them appearing 
to terminate in the plasma membrane. The fibres of the polar 
aster are longer than those of the central spindle. In these 
figures 3 and 4 there is a great deal of variation as regards the 
course of the fibres although in all cases they are extremely well 
developed. The fibres from the centers to the left in figure 3 
bend away from the plasma membrane, while those to the right 
for the greater part run towards the plasma membrane. The 
astral rays of the center nearest the tip of the ascus extend out 
in all directions, many of them running as far as the plasma 
membrane. In figure 4 the rays of the aster nearest the base of 
the ascus run to the edge of the spore plasm. The fibres of the 
aster nearest the tip spread out in all directions and could be 
traced to a considerable distance from their origin. In the two 
remaining asters most of the rays appear to end in the plasma 
membrane. The fibres in all these asters are extremely numer¬ 
ous, as can be seen from the figures. It is a conspicuous fact at 
this stage that the spindles are so arranged that the asters inter¬ 
fere as little as possible with each other. This condition is 
general at this stage. In the particular ascus here described the 
