1176 Wisconsin Academy of Sciences , Artsand Letters . 
spindles in figure 3 run almost at right angles to those in figure 
4 so that the four spindles form a four sided figure. The 
chromosomes are very small in this stage. In the late anaphase 
the chromosomes appears to he massed against the central bodies. 
The spindles elongate and the fibres of the spindle gradually dis¬ 
appear, while those of the polar aster become even more pro¬ 
nounced than during division, so that a section through the as- 
cus shows the large conspicuous red staining centers with the 
astral rays streaming out in all directions very prominently. 
The chromosomes are somewhat obscured by the centers. 
A little later the nuclear membrane forms around the chromo¬ 
somes and the nucleus becomes beaked. The nucleus at this 
time is still very small. The contents stain very densely. Sub¬ 
sequently the nucleus grows considerably and then the chro¬ 
matin and the nucleole can easily be distinguished. The astral 
rays develop still further. Figure 5 shows a longitudinal sec¬ 
tion of the ascus after the new daughter nuclei have reached a 
considerable size. The beak is conspicuous. None of the cen¬ 
tral bodies are turned toward the center of the ascus, but they 
lie irregularly arranged in the cytoplasm, as can be seen from 
the figure in which the beaks of the nuclei extend either toward 
the base, the apex or sides of the ascus. The cytoplasm is still 
of the same consistency as in the division stages. The asters 
are still larger. The rays of each aster extend out until they 
meet each other. This is especially conspicuous in figure 6. 
The fibres are distinct and those of one aster can easily be traced 
until they meet those of another. The cytoplasm is somewhat 
more darkly stained in the zone where the fibres meet. One 
might at first glance believe that the spores were already de¬ 
limited. In poorly stained sections where the asters are indis¬ 
tinct and the fibres cannot be traced very far from the centers it 
might appear that the spores are delimited by this zone formed 
by the meeting of the astral rays. But on careful observation 
it is plainly seen that this apparently limiting layer is nothing 
else than the region in which the rays of the different asters 
meet. These stages are comparatively frequent in my material 
and could very easily be mistaken for stages of spore delimita¬ 
tion. That the delimitation has not even yet begun is quite 
