1178 Wisconsin Academy of Sciences, Arts, and Letters . 
brane extends entirely around the spore plasm independently of 
the forming spore membranes. 
In later stages the fibres continue their growth backward and 
around the nucleus and delimit the spore. In figure 12 the 
membrane is not yet completed. The membrane of the spore 
can be plainly seen near the central body before that at the 
opposite end is visible. The spore near the tip of the ascus has 
been cut across at some distance from the center where the rays 
have not yet fused to form the membrane. The cut ends of the 
rays show plainly as a darker region. At this time the beaks of 
the nuclei still point in all directions in the ascus. 
Figure 13 is a little later stage in which the spore is com¬ 
pletely delimited. Some of the fibres of the plasma membrane 
as well as some inside the spore could still be distinguished. 
The nuclei and the centers are still connected with the spore 
membranes. The figure shows the beaks of the nuclei pointing 
in various directions as in the earlier stages just described. Ho 
membranes such as Faull described could be seen on the epi- 
plasm adjacent to the spore membrane. 
The beaks of the nuclei which up to the time when the spore 
membrane is completely formed were pointing in all directions 
in the ascus all come now to point in the same direction—toward 
the base of the ascus (figure 14) so that the central body in all 
cases is found on the inner end of the spore. That the mem¬ 
brane of the spore was formed independently of the ascus mem¬ 
brane is further proved by the facts shown in this figure 
since the spores have changed their position and the plasma 
membrane is wholly intact and not torn in places as we would 
expect to find it if it entered into the formation of the spore 
membrane. 
The spores now begin to elongate (figure 15) and come to lie 
more nearly side by side. They are found about fifteen to 
twenty microns from the tip of the ascus. The epiplasm is still 
finely reticulated and occupies for the most part the portion of 
the ascus between the spores and the apex. The center is at the 
part of the spore pointing toward the base of the ascus. It is 
still connected with the plasma membrane. The structure of the 
