Jolivette—Spore Formation. 
1179 
nucleus with its beak connected with the central body is still to 
be made out. The nuclei are considerably longer than broad in 
this stage. The nucleole is distinct. The chromatin is scat¬ 
tered in small masses in the nuclear cavity. The traces o£ the 
fibres have not entirely disappeared at this stage. 
The spores now become pointed at the end toward the base of 
the ascus (figure 16). It is apparent that this condition is due 
to growth. The persistence of the central body and nucleus in 
their position at the inner end of the spore would seem to indi¬ 
cate that they may be concerned in the growth of the spore at 
this point. The spores at this time lie side by side resembling 
in form a bunch of cigars. Although this growth process has 
taken a comparatively long time, which is shown by the numer¬ 
ous different stages that can easily be found, the nucleus is still 
beaked and the central body is in contact with the spore mem¬ 
brane. The astral rays have entirely disappeared by this time. 
The epiplasm is still in the same condition as before. Finally, 
however, after the spore has reached a considerable length the 
nucleus with the central body is drawn back from the wall. 
Figure 17 was drawn from a cross section of the ascus which 
cuts through the spores in the region of the nuclei after the 
latter have moved back to the middle of the spores. The cyto¬ 
plasm here appears a little more coarsely reticulated than in the 
earlier stages. The nucleus is usually slightly elongated in the 
long axis of the spore at this time, becoming more spherical in 
shape a little later. 
The spores increase in length, the cytoplasm takes on a 
spongy appearance, the nuclei divide karyokinetically and cross 
walls are put in so that the spores become several celled. The 
walls of the spores become thicker. The spores now vary in 
length and are less regularly arranged than they were at an 
earlier stage, although they still occupy the distal end of the 
ascus. 
General Discussion. 
As described above the polar asters of the third division in 
the ascus of Geoglossum glabrum Pers. grow until they 
come into contact with each other and form a definite region, 
