1182 Wisconsin Academy of Sciences, Arts, and Letters. 
round up due to surface tension. But the ascus membrane* 
where it is impossible to trace it past the spores, can be seen to 
thin out gradually to a delicate film on either side of the spore 
(fig. 11, point b). In some cases these films on either side of 
the spore can he traced almost to the point of union of the two. 
If the ascus membrane was cut into during the formation of the 
spore membrane these edges could not maintain themselves hut 
would necessarily draw hack and round off. In still other cases 
the ascus membrane with a thin layer of epiplasm can just 
barely be traced outside the spore membrane. 
A further proof of the formation of the spore membrane in¬ 
dependently of the ascus membrane is that, in all stages where 
the spores are completely delimited and have started to change 
their position, the ascus membrane is still entire. Figures 13,. 
14, 15, 16 and 17 showing completely delimited spores show no 
cases of incomplete ascus membranes. 
In Geoglossum glabrum Pers. the nuclei with their central 
bodies do not always point toward the median line of the ascus. 
The axes of the spindles of the third division show no tendency 
to lie in the transverse axes of the ascus; they may lie at any 
angle. Figures 3 and 4, examples of this stage, show this 
plainly. In this case two of the spindles are practically at right 
angles to the other two. The arrangement appears to be such 
that the asters interfere very little with each other. Accord¬ 
ingly the nucleus with its central body may point toward the' 
distal or proximal end of the ascus or at almost any angle with 
the wall. Figures 5, 6, 7 and 8 afford striking examples of 
this condition. The nuclei are not arranged regularly with 
their beaks at right angles to the ascus wall but are pointed in 
almost any direction except toward the center of the ascus. 
In these stages the interastral zones are quite conspicuous as 
described and it is quite possible Faull (13) took this zone for a 
limiting layer of the spores. As noted above, in poorly stained 
preparations where the asters were not well differentiated the 
interastral zone might at first glance appear to be a limiting 
layer. Such preparations may have misled Faull (13). The 
meeting of the fibres in the zones above described might be in- 
