PHYSIOLOGY OF PEPSIN-FORMING GLANDS. 
709 
Fig. 5. X 290. End-tubes of oesophageal gland. From Frog (September, 1879) 
forty-five hours after feeding with large piece of sponge. The granules are 
much diminished in size, and form only a narrow zone around the enlarged 
lumen. Several end-tubes in this specimen had lost all their granules. 
Fig. 6 («). X 330. End-tubes of oesophageal gland. The granules have disappeared 
from the cells, except at one place where a few small granules border the 
lumen. From Frog (September, 1879), twenty-five and a half hours after 
feeding with sponge. In no other Frog which I have observed did the 
granules disappear in so short a time. In this and the preceding figure the 
striation of the cells is much too distinct. 
Fig. G ( b ). X 410. Same specimen as preceding after three months’ stay in dilute 
glycerine. The striation and the very fine granules which are often seen 
in the clear zone are here brought out. 
Fig. 7. X 350. End-tube of oesophageal gland. Frog (September, 1879) fed with 
sponge; after twenty-two hours sponge removed; 1*5 cub. centirn. strong 
aqueous solution of peptone injected under skin of back; animal killed twenty- 
six hours after this. The granules are comparatively few, and although more 
numerous in the inner portion of the cells, occur also in their outer portion. 
Fig. 8. X 340. End-tubes of oesophageal gland. Tissue placed in absolute alcohol 
for twenty-four hours, then in dilute carmine for twenty-four hours. Shows 
stained non-granular and unstained granular zones. Frog (September, 1879) 
fed with sponge thirty-five hours ; sponge then vomited ; animal killed ten 
hours after. 
Fig. 9. X 410. Anterior oxyntic gland. Hungry state. The cell outlines are 
scarcely or not at all to be made out ; the granules are distinct, numerous, 
and scattered throughout the cells. The nuclei and cell-substance is very 
slightly stained. Frog (July, 1879) killed five days after a good meal. 
Fig. 10. X 580. Posterior oxyntic gland. Three hours’ digestion. The granules 
are smaller than normal, and have disappeared from the inner border of the 
cells, leaving a small homogeneous zone. The nuclei and cell-substance stain 
darker. The nuclei are placed in the outer portion of the cells, in this speci¬ 
men indeed more so than usual. The corkscrew-shaped lumen is obvious. Frog 
(April, 1879) several days’ hunger, then fed with worm, killed in three hours. 
Fig. 11. X 370. Posterior oxyntic gland. Twenty-five hours after heavy meal. 
Frog (April, 1879) several days’ hunger, fed with four worms ;. killed twenty- 
five hours after; stomach still distended with debris of food, although a con¬ 
siderable amount fills out the intestine. The cells bulo-e out into the larue 
o o 
lumen ; the homogeneous inner zone is distinct. 
