1306 Wisconsin Academy of Sciences, Arts, and Letters. 
I his investigation was undertaken with the following pur¬ 
poses in view: first, to study, criticise, and, if possible, improve 
some of the bacteriological methods occasionally used in milk 
work; second, to try out certain of these methods on different 
classes of milks, with special reference to the ease and reliability 
with which they may be used to differentiate good (or safe) 
from bad (or dangerous) milks; third, to determine, in a general 
way, the conditions of the milk supply of a large city by means 
of the technique selected. 
II. BACTERIOLOGICAL METHODS OF MILK ANALYSIS. 
A. Collection of Samples. 
Description of Collecting Case. It seemed necessary to 
have considerably more milk than could be collected in a test- 
tube such as is provided for in the milk-collecting outfit of the 
Boston Board of Health, or that used bv Miss Schroeder in her 
work for the New York City Board of Health work.* 
Four ounce bottles with metal caps which screw on and which 
are ordinarily known as oil sample bottles were used. In these 
bottles about one hundred cubic centimeters can be placed and 
still leave plenty of room for shaking. 
As a carrying case a fiber handbag was secured, 8 inches wide, 
8 inches high, and 16 inches long. Inside of this a copper box 
was fitted, somewhat smaller than the bag, and so arranged that 
there would be an air space all around it of an eighth to a quar¬ 
ter of an inch. The box was divided into three compartments by 
partitions, one to contain eighteen of the above bottles, held in 
place by a metal rack. This rack can be collapsed if necessary, so 
that pint milk bottles can be carried. Another compartment is 
for ice, and the third, rather narrow and the full length of the 
box, was fitted with a pipette case. This was held up to the top 
by lugs, leaving an empty space below into which the used pi¬ 
pettes can be placed. The pipettes used were sixteen inches long 
and made of glass or aluminum tubing, having about % of an 
inch as inside diameter. The general arrangement is shown in 
the accompanying figure. Fig. 1, Plate XCIV. 
* Jour. Infect. Ris., 11, p. 2. 
