DR. C. A. MAC MUNjST ON THE CHROMATOLOGY OF ACTINIAE. 
649 
red solution filtered, through asbestos ; in this solution the acid hsematoporphyrin 
spectrum was plainly seen, much more plainly than in the case of other Actiniae, 
so that readings could be easily taken, the first band extended from X 613 to 
X 596'5, and the second from X 566 to X 551 ; here a third feeble shading from X 524'5 
to X 501 was also seen. On comparing these readings with those got in former cases 
the agreement is tolerably close, especially when we take into consideration the fact 
that in the case of Actinia mesembryanthemum the bands of a solution diluted with 
rectified spirit were measured, while here the pigment was dissolved in pure sulphuric 
acid. In the latter case the pigment could also be converted into alkaline hsemato¬ 
porphyrin. 
If a Bunodes crassicovnis be taken whose tentacles show the aetiniohsematin band, 
and if the tentacles be extracted with glycerin, the solution shows a much broader band 
between D and E than that of Actiniochrome ; then on adding caustic soda the band 
is made fainter, and on adding sulphide of ammonium the hsemochromogen bands 
are seen with distinctness, but if those tentacles which show Professor Moseley’s 
Actiniochrome are treated in the same way a negative result is obtained. 
Biliverdin appears to he present in the mesoderm and in other green parts. 
In some specimens I found actiniohcematin and actiniochrome mixed together, and 
in the glycerin extract of some tentacles two bands nearer the violet, as shown in 
spectrum 7, Chart II. I do not think these bands belong to a lipochrome (= lutein) 
as they are not found in solutions which dissolve the lipochromesA Similar bands 
were seen in the case of other anemones, as will be seen by referring to the spectra 
figured. 
Hence in Bunodes crassicovnis we find aetiniohsematin with tolerable constancy, 
occasionally actiniochrome and also biliverdin, besides the lutein-like pigments. In the 
ectoderm as well as in the endoderm, and sometimes in the tentacles, aetiniohsematin 
is present. In none of the specimens w r ere “ yellow cells” present, and by no other 
solvents except glycerin, and alkaline and acid alcoholic solutions, could any pigments 
be got into solution. 
Bunodes ballii (large variety).—The specimens which I have examined came from 
Weymouth. The body wall was dotted with red, the tentacles and body at their 
base were green, and the tentacles presented a contrast in their opacity to the Actinise, 
examined supra. In other specimens the body wall (ectodermal layer) was red or 
brownish-red, the upper fifth or so greenish, with rows of white dots running from 
base to disc. 
The tentacles gave a number of bands, (see spectrum 10, Chart II.,) which showed 
* “ Lipockromes ” is a name proposed by Krukenberg for a class of pigments some of which were 
formerly known as luteins. See his ‘ X^ergleichend-physiologische Studien,’ 1880—1882, and ‘ Grundziige 
einer vergleichenden Physiologie der Fai'bstoffe und der Farben,’ 1884. I hope to have more to say on 
this point in a future paper. 
