650 
DR. C. A. MAC MUNN ON" THE CHROMATOLOGY OF ACTINLE. 
that a chlorophyll-like # pigment was present in them. The interior parts—mesenteries, 
&c., gave the same spectrum. The ectoderm in the redder parts gave a faint band in 
green, as shown in spectrum 11, Chart II., while in its green parts—with few excep¬ 
tions—the same spectrum as that of the tentacles could be detected. On extracting 
the tentacles with absolute alcohol for a couple of days and filtering, an orange- 
coloured solution, with a distinct red fluorescence, was obtained, and this gave 
spectrum 12, Chart II. These bands gave the following readings: 1st band, A. 675, 
to X 657, 2nd band, X 642‘5 to A.629, and 3rd band, X 595 to X579, the light being com¬ 
pletely absorbed at X543. On treatment with one drop of nitric acid the spectrum 
changed completely, a darker band appeared placed over a lighter one, the whole 
compound band extended from X 675 to X647, and its dark part from X 675 to X665 ; 
another also before D from X 623 to X596’5, light being completely absorbed at X 521’5. 
Spectrum 13, Chart II. A feeble band also was seen after D, not shown in the map. 
The first alcohol extract left the tentacles a vivid green, but on repeated extraction 
with alcohol the colour became much diminished, and the second alcohol extract did 
not differ from the first. 
On extracting the interior parts of this anemone with absolute alcohol and filtering, 
an orange-coloured solution was obtained, and this gave a very faint red fluorescence 
and a faint spectrum, not differing from that of the alcohol solution of the tentacles, 
after allowing for the smaller amount of pigment present in the solution. In a thin layer 
of the solution a feeble band was seen extending approximately from X 509 to X 484'5. 
On extracting 1 the ectoderm with absolute alcohol and filtering, an orange-coloured 
solution was also obtained, giving the same spectrum as the parts referred to, spec¬ 
trum 14, Chart II., and having a red fluorescence. These bands read as follows: 
1st from X 675 to X 657, 2nd from X645 to X 629, and 3rd from X 595 to X580'5, 
the spectrum being extinguished at X 551. 
On treating this last alcoholic solutionf with caustic potash the spectrum changed 
completely , as shown in spectrum 15, Chart II. The result obtained by extracting 
the ectoderm with rectified spirit and caustic potash and filtering was no less striking; 
the resulting solution was distinctly green, and gave the spectrum shown in 
spectrum 16, Chart II. The first and second band read: 1st from X 649 to X627, 
2nd X 609 to X585. In a thin layer a third became detached at X 492'5 to X475. No 
heemochromogen bands could be obtained. At the same time I believe the red 
portions contain a small amount of actiniohmmatm, its presence being masked by 
* In the list of chlorophyll-containing animals, enumerated by Professor Lankester, in the second 
English edition of Sachs’s ‘ Botany,’ the word chlorofucin is placed after Anthea cereus. I have accord¬ 
ingly introduced the spectrum of chlorofucin from Fticus serratus (Linn.), and there is seen a remarkable 
likeness, if not an identity, between it and the pigments of the above, S. bellis and A. cereus. 
Spectra 8 and 9, Chart II. Sorby was the first who showed the presence of chlorofucin in A. cereus. 
Pi’oc. Royal Soc., No. 146, vol. xxi., 1873, p. 454. 
f Or that of the tentacles. 
