G52 
DR. C. A. MAC MUNN ON THE CHROMATOLOGY OF ACTINI2E. 
But although the examination of the solid parts of this species is so unsatisfactory, 
I got distinct evidence of the presence of a heematin-yielding pigment. On digesting 
portion of the ectoderm of brown specimens in rectified spirit and caustic potash a 
reddish-yellow solution was obtained which gave a band at D, and a shading at the 
blue end of green, the former from A 611 to A 579. And then, on adding ammonium 
sulphide, this band did not disappear, but a faint band resembling the first reduced 
haematin band was distinctly seen, spectrum 3, Chart III. On digesting the lining of 
the body cavity of the same specimens in the same solution, a very feeble band at D 
was detected, and on adding ammonium sulphide the result was the same as in the 
case of the ectoderm. The tentacles were digested in absolute alcohol, and the almost 
colourless solution gave a feeble band from A 505 to A484‘5, unchanged by caustic 
potash. The examination of the endodermal parts of white specimens gave the same 
result as above. 
In a large brown Sagartia dianthus I also got distinct evidence of the presence of 
actiniohsematin. The reddish-yellow filtered extract, obtained by digesting the ecto¬ 
derm in rectified spirit and caustic potash, showed a band from A 623 to A 585, and on 
adding sulphide of ammonium two hcemochromogen bands were seen, of which the 
first (approximately) measured from A564'5 to A 554‘5. The same ectoderm yielded 
nothing to absolute alcohol, nor did the same part of an orange S. dianthus to the 
same solvent. 
Sagartia viduata. —The few specimens which I had an opportunity of examining 
were striped with brown and white, and no well-marked band could be detected 
either in the ectoderm or endoderm. A faint shading in green was the only 
noticeable appearance. 
On extracting the ectoderm for twenty-four hours with rectified spirit and caustic 
potash a yellowish solution was obtained, and this gave spectrum 4, Chart III. 
In this spectrum the first band read from A 657 to A 631, and the second from A 611 
to A 582 (?). If this result is compared with the result of the examination of 
Bunodes hallii a resemblance is noticed. Faint traces of hsemochromonen were 
detected on adding ammonium sulphide.* 
Sargatia parasitica. —In some specimens of this species—of a brown colour, externally 
striped with yellow and being internally a reddish-yellow colour,—no distinct band 
could be seen. In the brownish-red base no band could be detected, but both by the 
ectoderm and by the latter the violet end of the spectrum was strongly absorbed. 
The tentacles were white, but here and there dotted with a purplish pigment which 
showed no band. In some specimens the circumoral part had a purple-red colour, 
which gave an ill-defined band between D and E. Microscopically two kinds of 
pigment could be seen in them—brown and purple-red. Corresponding to these 
appearances was the result of extracting the various parts with solvents, as by their 
* I believe the above chlorophyll-like spectrum was due to presence of “yellow cells,” but I failed to 
detect them in the specimens examined. 
