670 Wisconsin Academy of Sciences, Arts, and Letters. 
since, in a somewhat later stage, the whole of the region be¬ 
tween the dark violet line and the violet starch layer takes the 
violet stain and becomes the outermost violet layer of the grain. 
The dark violet line in but few instances was seen to pass 
around the hilum, but as the sides of the grain are approached 
the line becomes narrower and finally disappears. As has been 
pointed out, the peripheral layer stained by orange is trans¬ 
formed in the growth of the grain into violet-staining layers. 
This may be due to a condensation of the carbohydrate mate¬ 
rial, brought about by the abstraction of water, or to' a more 
deeply seated chemical change. It is possible that this orange- 
staining substance is already carbohydrate material, which has 
been brought inside the leucoplast and which is then trans¬ 
formed by the addition of water into starch and gains the capa¬ 
city to fix the violet stain. This would seem to be a more 
natural assumption than that starch can show such a variable 
reaction to the same stains, as assumed by Salter. 
In the development of the cell plate in root tips, the equa¬ 
torial zone was found by Timberlake (40, p. 97) to become 
filled with a substance that stains strongly With the orange of 
the triple stain. This substance appears to be entirely homo¬ 
geneous and with ruthenium red or iron haematoxylin appears 
colorless while the cell wall is stained. Timberlake says: 
“The similarity of this substance to that of the cell wall, to¬ 
gether with its presence in the region of the spindle in which 
the cell wall appears later, I have taken to signify the presence 
of a carbohydrate substance destined for the formation of the 
new cell wall.” 
In the germinating seeds of Coix lacryma, the walls of the 
endosperm cells disappear after the young plant has attained 
some size, and are apparently used to nourish the growing 
plant. These walls, while in process of solution, take the or¬ 
ange stain when the triple stain is used. 
Preparations which I have studied show that the cellulin 
bodies in the cells of Saprolegnia , which are carbohydrate in 
nature, take a bright orange when stained by the triple stain. 
TSToll (27) has shown that these bodies in the Siphoneae are 
