534 
Miller and Carlton—The Cat's Kidney. 
THE RELATION OF THE CORTEX OF THE KIDNEY TO THE ENTIRE AREA 
OF SECTIONS TAKEN FROM KIDNEYS HARDENED IN MUELLER’S 
FLUID. 
Four pairs of kidneys were hardened in Mullers fluid followed 
by alcohol. The cats were chloroformed and killed by bleeding. 
All fat was removed and vessels cut at hilum. The volume of 
each kidney was found by displacement in water. The neys 
were hardened in Mullers fluid; washed in running water and 
eventually placed in 95 per cent, alcohol. They were then im¬ 
bedded in celloidin and sections cut £5/* thick. Before placing 
the kidneys in Mullers fluid, the kidneys were cut in three 
four pieces each—the right longitudinal and the left transverse 
to the long axis, or vice versa. No record was kept of the 
thickness of these pieces and the volume of the cortex could not 
be computed for this reason. A section from each one of these 
pieces was put between two slides and fastened in the table so 
that the glass was on a level with the surface of the table. The 
area of the entire section and of the medulla and boundary zone 
was then found with a planimeter. The results are given in 
Table VII. The sections are either longitudinal or transverse 
and when the line of section passes through the pelvis it is in¬ 
dicated by a *. The areas are in square decimeters. In the 
last column the volume of each kidney is given, and the direc¬ 
tion in which it was cut before hardening in Mullers fluid indi¬ 
cated by L or T below the volume. The volume is given in 
cubic centimeters. The Mullers fluid does not appear to have 
caused as much shrinkage of the cortex as the alcohol. 
