287 
Pollen Mother-cells of Lilium candidnm , Linn. 
In this way, then, the globules reach the cytoplasm of an adjacent 
mother-cell, where they at first appear as smaller or larger roundish deeply 
staining masses, each one of which retains its connexion with the synaptic 
knot of the parent nucleus by means of a very fine thread passing through 
the cell-wall (Figs. 1-5). These connecting threads, which persist for 
a considerable period, judging from the frequency with which they are 
found, also take up ‘ chromatin 5 stains, hen e their presence can easily be 
demonstrated in preparations stained with iron-haematoxylin, or with the 
combination stains of Flemming or of Breinl. The extruded globules 
frequently assume a pear-shaped outline and occasionally 4 secondary 
globules ’ are protruded from their periphery (Fig 5). These bodies are 
always surrounded by a perfectly clear zone ; the precipitation-membrane 
delimiting this clear space from the surrounding cytoplasm appears to 
be of the same nature as that which surrounds the nuclear vacuole and 
in both cases is very indistinct. In preparations treated with the above- 
mentioned cytological stains the nuclear vacuole appears to be bounded 
by a close aggregation of cytoplasmic fibrils (Figs. 1 and 4). 
At a somewhat later stage, when the bodies have lost their connexion 
with the parent nucleus, they appear, in carefully stained preparations, 
as isolated deeply stained globules scattered throughout the cytoplasm 
of the invaded cell. Great difficulty was experienced in following the 
subsequent history of the extruded material, all trace of which is very 
quickly lost. It appears to be ultimately absorbed by the cytoplasm 
of the invaded cell. 
In two anthers which had been fixed in Hermann’s solution the 
condition of excessive 4 chromatin ’ extrusion shown in Fig. 3 was observed. 
These bodies are also given off while the nucleus is coming out of 
synapsis and during the inception of the 4 hollow-spireme 5 stage. That 
these bodies are actually extruded during the later synaptic stages, and are 
not merely the remains of those given off at complete synapsis, is shown by 
the fact that at these later stages the globules are usually small and rounded 
while the connecting threads are very well defined. Moreover, these con¬ 
necting threads always persist until the walls of the mother-cells begin to 
separate. During these later stages of meiosis the elimination of nuclear 
material is usually less pronounced (Figs. 4 and 6). The extruded material 
often presents a beaded or granular appearance (cf. Digby ( 4 ), PI. XXXIV, 
Fig. 16), which is probably due to a difference in 4 chromatin ’ concentration 
in the substance of the 4 bodies but nothing approaching the condition of 
a spireme, as described by Gates for Oenothera gigas, has been observed in 
Lilium candidtim. 
4 Chromatin * extrusion into an adjacent tapetal cell was never observed 
in our preparations, although lateral extrusion from one mother-cell into 
two neighbouring mother-cells, or lateral extrusion from two mother-cells 
