of locating Sugars in Plant Tissues . 375 
When prepared the tubes were corked, heated for one hour at 98° C., 
and examined on cooling and subsequently from time to time. 
The results given are shown below : 
• 
On removal. 
After 1 day. 
After 3 days. 
After 4 days. 
Cane sugar, i % 
Clear yellow. 
Slightly turbid. 
Little change. Thin 
film at top. 
Much the same. 
» „ 10 % 
Clear yellow. 
Slightly turbid. 
More turbid. 
Slight deposi¬ 
tion of osazone 
crystals. 
Dextrose, i % 
Clear yellow. Crys¬ 
tals had formed 
half an hour later. 
Plenty of crystals. 
Abundance of small 
osazone crystals 
forming a precipi¬ 
tate at bottom of 
tube. 
Much the same. 
Levulose, i % 
Practically a solid 
mass of osazone 
and glycerine. Tube 
invertible within 
5 minutes. 
Much the same. 
Much the same. Crys¬ 
tals, being larger 
than those given by 
dextrose, were more 
uniformly distri¬ 
buted. 
Much the same. 
Maltose, i % 
Clear yellow (as i % 
cane sugar). 
Slightly turbid. 
Much the same. 
Much the same. 
„ 10 % . 
Darker yellow, but 
quite clear. 
Slightly turbid. 
Much the same. 
Much the same. 
In order to follow the changes microscopically drops of the mixtures 
were also mounted on slides having depressions ground in one surface, and 
the cover-slips were attached with wax mixture. 1 
After | hour. 
After 1 day. 
After 3 days. 
After 4 days. 
After 45 days. 
Cane sugar, i % 
Minute yellow- 
brown globules. 
Little change. 
Little change. 
Little change. 
No osazone crys¬ 
tals. 
» „ 10% 
More numerous 
globules than 
in 1 %. 
Moderate number 
of crystals. 
Increase in number 
of crystals. 
Abundance of 
crystals though 
much less than 
in 1 % dextrose. 
Abundant spheri¬ 
cal clusters of 
crystals, dense, 
almost woolly, 
outline not 
sharp. 
(= ( d.H type.) 3 
Dextrose, i % 
Crystallization set 
in almost at 
once. Spherical, 
rather feathery 
aggregates of 
fine, acicular, 
short crystals. 
( = ‘«C type.) 2 
Little change. 
Plenty of mode¬ 
rately large crystal 
clusters with great 
numbers of very 
much smaller, less 
dense ones present 
between them. 
Much the same. 
Much the same. 
1 Minute crystals may be present in the glycerine, and although these may not be visible to the 
naked eye, they become obvious under the microscope. 
2 Cf. PI. XVII, Fig. 2. 
Cf. PI. XVII, Fig. 3. 
