380 Maugham.—Observations on the Osazone Method 
When 
examined. 
After 
18 hours. 
After 
42 hours. 
After 
4 days. 
After 
6 days. 
After 
21 days. 
Levulose. 
Tubes, o-1 % Quite clear. Straw-coloured. Tubes. 
i*o % No further change. 
Slides. o-i% Good crop of short, small, Slides. 
acicular crystals, mostly 
separate, but some in 
sheaves or groups. 
Dextrose. 
o-i % Quite clear. Straw-coloured, 
i-o % No further change. 
o*i % A distinctly smaller crop of 
crystals than with o*i % 
levulose. Crystals con¬ 
fined to edges of depression 
—not in latter itself, as 
with levulose. Individual 
crystals indistinguishable 
from those of levulose. 
1 -o % Much as with levulose. 
i-o % Dense formation of crystal 
clusters, between which 
the liquid looked turbid. 
Crystal clusters pale, 
almost transparent, and 
not very clearly defined. 
Some larger sheaves also 
present. 
Tube, o-i % Still quite clear to naked 
eye. A drop removed and 
examined microscopi¬ 
cally, however, showed 
a few small crystal clus¬ 
ters and one or two 
larger ones. Some almost 
transparent, flocculent 
masses or films of irregu¬ 
lar outline also micro¬ 
scopically visible. 
Slides, o-i % Very good crop of crystals, 
separate or in sheaves, 
throughout the liquid. 
I'02 
' Dense mass of spherical 
crystal clusters. The 
radiating crystals most 
clearly defined in central 
portions of each cluster, 
while their outer ends 
could not readily be 
delimited from those of 
crystals of adjacent clus¬ 
ters. Optically indis¬ 
tinct—crystals apparently 
not properly separated as 
such from matrix. 
Tube, o-1 °/. 
| Precipitation of crystals 
commencing in upper 
portion of liquid. 
Slides. 
Much as before. 
Tube, o-1 % 
The crystals viewed from 
above end of tube showed 
a radial distribution. 
Slides. 
Much as before. 
Tube, o-1 % 
’ Increased crop of crystals. 
Tube. o« 1 % Still quite clear to the eye. 
A drop examined micro¬ 
scopically showed dis¬ 
tinctly fewer crystals than 
with o* 1 % levulose. Floc¬ 
culent masses as with levu¬ 
lose — possibly osazone 
about to crystallize. 
Slides, o-1 % An increased number of 
crystals present practically 
all round edge of depres¬ 
sion, but not yet in latter. 
Similar in appearance to 
levulose, but much less in 
quantity. 
1*0 % Much as with levulose. 
Tube . 0.1 ^ 
' Smaller amount of crystals 
in course of precipitation 
than in levulose. 
Slides. 
Much as before. 
Tube, o-i °/ ( 
' As with levulose, but in 
smaller amount. 
Slides. 
Much as before. 
Tube. o*i % 
| Much smaller crop of crys¬ 
tals than with levulose. 
