382 Maugham — Observations on the Osazone Method 
with pure glycerine in ten different proportions, viz. 1 per cent., 2 per cent., 
3 per cent., 4 per cent., 5 per cent., 6 per cent., 8 per cent., 10 per cent., 
15 per cent., and 20 per cent. 
As in previous experiments drops of the mixtures were mounted on 
slides, sealed and examined microscopically at intervals over a long period. 
The behaviour of maltose phenylosazone under these conditions differed 
markedly from that of the osazones of dextrose and levulose. 
No rapid re-crystallization occurred, but the preparations showed a 
series of changes which took place with extreme slowness. 
After the tubes had cooled, their contents ranged in colour from 
pale straw to very deep brown. 1 With the rise in concentration of the 
osazone the viscosity of the mixture increased, the 20 per cent, mixture 
being almost invertible. 2 Owing to the opacity of the higher mixtures 
satisfactory observations could not be made on them in the tubes. 
In all the less opaque tubes a slight increase in turbidity was noticed 
for a few days from the commencement of the experiment. 
After four days the tubes up to 6 per cent, showed a somewhat less 
opaque top layer. 
Observations made upon the prepared slides showed that suspended in 
the fluid medium were numerous very minute globules of yellowish-brown 
syrup. In any one slide these globules varied in size over a not very wide 
range. Their number increased more or less in proportion to the concentra¬ 
tion of the osazone. 
On examining the preparations by dark-ground illumination and with 
a Zeiss 16 mm. objective and x 18 compensating ocular the presence of an 
immense number of minute particles could be detected. These were best 
seen in the weaker mixtures, and according to their size they scattered rays 
of different wave length, and so appeared as bright red, orange, green, &c., 
points of light. 
The number of points of light which could be detected by dark-ground 
illumination was much greater than that of the globules visible by weak 
transmitted light. 
It was possible to observe an extremely slow motion of the points of 
light in the glycerine, i. e. slow, compared with the motion of colloidal 
particles in a metallic hydrosol. 
The minute yellowish-brown globules showed a fortuitous arrangement 
at first, but gradually, and more obviously in the higher concentrations, 
they became grouped to form irregular clusters leaving spaces more or less 
free. Many of the globules cohered, and some by coalescence gave rise to 
larger ones. By the fourth day this grouping of globules into chains, &c., 
1 Probably due to small amounts of aniline or decomposition products present as impurities. 
2 Cf. the hexoses, pp. 379-80. It is possible to prepare emulsions of oil and soap having 
a consistency allowing of cubes being cut out of them. Hatschek (’ 13 ), p. 22. 
