390 Mangham.—Observations on the Osazone Method 
There is a slight possibility of mistaking for the osazone of maltose 
other crystals such as those of salts of phenylhydrazine, or of the oxidation 
products of glycerine should these be formed. 
In conclusion, it may be said that when using Senft’s reagent it is 
advisable to re-examine the preparations from time to time over a period of 
at least four months before attempting to draw conclusions from them. 
Literature cited. 
Armstrong, E. F. (’ 12 ): The simple Carbohydrates and the Glucosides. 
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- (’ll): On the Detection of Maltose in the Tissues of certain Angiosperms. New 
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description of photomicrographs in plate XVII. 
Illustrating Mr. Mangham’s paper on the Osazone Method of locating Sugars in Plant Tissues. 
Fig. 1. Osazone crystals yielded by 1 per cent, of levulose in Senft’s reagent after heating for 
one hour. Photographed four months after heating, x 114. 
Fig. 2. Osazone crystals yielded by 1 per cent, of dextrose in Senft’s reagent after heating for 
one hour. Photographed four months after heating. Note feathery edges of crystal clusters, x 114. 
