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Y. Further Observations on Enterochlorophyll, and Allied Pigments. 
By C. A. Mac Munn, M.A., M.D. 
Communicated by Professor M. Foster, Sec.R.S. 
Received April 21,—Read April 30, 1885. 
[Plates 9, 10.] 
Lsr a paper, read before tbe "Royal Society in 1883,* I described the results of an 
examination of tbe so-called “bile” of invertebrates, and proved that the alcohol 
extracts of the “ liver,” or other appendage of the intestine answering to it, showed 
a spectrum so like that of vegetable chlorophyll as to have led me to conclude that 
no essential difference exists between the spectrum of enterochlorophyll and plant 
chlorophyll. 
At that time I could not decide the points which are now considered. 
The object of the present investigation was to determine :—(1) Whether entero¬ 
chlorophyll is due to the presence 0 f symbiotic algse or not; (2) whether it is an 
immediate food-product and merely an instance of the intracellular digestion of food 
chlorophyll; (3) if it is not derived from either of these sources, is it built up by and 
in the liver of the animal yielding it? (4) in what points does it differ from plant 
chlorophyll and the chlorophyll of Spongilla? 
I believe I can prove the absence of symbiotic algse, the absence of food-products, 
the animal origin of the pigment, and that it yields, at least in some cases, similar 
decomposition-products to plant chlorophyll. This evidence is not based on spectro¬ 
scopic examination only, but also on the study of the morphology of enterochlorophyll 
and on the absence of starch and cellulose. 
At the same time I do not wish to appear too confident as to the purely animal 
origin of the pigment in all cases. 
Spectrum of Chlorophyll in a Living Leaf and in an Animal containing Chlorophyll 
built up by itself 
What is the spectrum of chlorophyll in a living leaf? To enable one to reply to 
this question a suitable method of examination must be adopted, and I may say at 
* Proc. Roy. Soc., vol. 35 (1883), p. 370. 
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