242 DR. C. A. MAC MURAT ON ENTEROCIILOROPIITLL, AND ALLIED PIGMENTS. 
rayed Solaster the dominant band of an absolute alcohol solution of the radial coeca— 
which was yellow and fluoresced red—extended from X 669 to X 657. Other speci¬ 
mens were examined with the same result, their radial coeca furnishing only a small 
amount of enterochlorophyll. No enteroheematin could be detected; and in none of 
their integuments was hsematoporphyrin found. A full account of the integumental 
colouring matters is reserved for another communication. 
Uraster rubens. —A great number of this species have been examined, and the 
results obtained are so important in deciding the question as to the animal origin of 
enterochlorophyll, that I think it necessary to give the results in greater detail than 
in other cases. 
(1) Uraster with a nodulated integument.—A filtered alcohol extract of the coeca 
was a dull sap green, and showed a red fluorescence and two well-marked bands, and a 
feeble third in a deep layer ; also in a shallow layer an ill-defined band at the blue end of 
green. The bands read :—1st, X 669 to X 640 ; 2nd, X620'5toX 591. With nitric acid 
the spectrum changed, and the bands read :—1st, X 665 to X 640 ; 2nd, X 618 to X 591 ; 
3rd, X 579 to X 557 (?). The great breadth of the band in red is here noticeable. 
(2) Uraster with a red integument.—Here the alcohol extract of the radial coeca 
was greenish, fluoresced red, and gave the same kind of band in red as (l), but after 
this solution had stood twelve hours the band in red could be no longer seen, and the 
colour of the solution had changed to orange-yellow. The residue left after evapora¬ 
tion of the alcohol was a pale, yellowish-brown colour, and dissolved in chloroform 
showed one faint band in the blue part of green. Hence it would appear that the 
enterochlorophyll in this case spontaneously changed into another colouring matter; the 
amount of material was, however, too small to enable me to determine what this 
colouring matter w r as. 
(3) A violet Uraster. —The radial coeca yielded when extracted with alcohol a deep 
gamboge-yellow coloured solution, showing a badly-marked band in red, corresponding 
in position with the above-mentioned band, but showing a strong absorption of the 
violet end of the spectrum up to E. In a thin layer a broad ill-defined shading from 
X 516 to beyond X 475 became detached. On adding nitric acid a distinct blue colour 
was produced, the band in red moved nearer the violet, and the broad shading could 
be no longer seen. Caustic soda did not cause this shading to disappear. There is no 
doubt that these coeca contained a colouring matter which belongs to the lipochromes, 
and I believe it is more like K&hne’s rhodophan or xanthophan than anything else. 
Now, as we find in the integument a similar pigment, there can no longer be a doubt 
that the integumental pigment (as I formerly showed) is prepared in the radial coeca. 
(4) A reddish-brown Uraster. —The radial coeca when extracted with alcohol 
furnished a dark-yellow solution with a red fluorescence. This showed spectrum 17, 
Chart I., the result of combining the spectrum of a deep and shallow layer. The 
bands read :—1st, X 667 to X 649 ; 2nd, X 611 to X 593 ; and 3rd, X 541’5 to X 529’5. 
