DR. C. A. MAC MUNN ON ENTBROCHLOROPHYLL, AND ALLIED PIGMENTS. 243 
There was a 4th band, which, after adding caustic soda, became more distinct, and 
read about X 505 to X 484'5 ('(). 
(5) The coeca of a brown Uraster were also extracted with absolute alcohol, the 
extract added to that obtained from the coeca of (4), and saponified by Hansen’s 
method. The extract from the coeca of (5) agreed in its spectrum with that of (4). 
The result of this experiment will be described under “ Saponification.’ ’ 
(6) An orange and brown specimen of Uraster.- —-The dirty green radial coeca were 
extracted with absolute alcohol, and furnished an orange-coloured solution with a red 
fluorescence, which showed a spectrum similar to 17, Chart I. The bands read 
approximately : 1st, X 667 to X 651'5 ; 2nd, X 615’5 to X 593 ; 3rd, X 543 to X 532 (?), 
and a 4th, about X 511‘5 to X 486’5. 
(7) A brown Uraster, with greenish coeca.—These formed in absolute alcohol a 
yellow solution with a greenish-brown tinge and a red fluorescence. The bands read : 
1st, X 669 to X 647 ; 2nd, X 613 to X 593 ; 3rd, X 540 to X 530’5. The 4th band 
could not be read distinctly, but appeared to be X 5115 to X 475 (?), probably formed 
by the coalescence of two bands. 
If any of these last three solutions were treated with nitric acid it became greenish, 
and the bands read: 1st, X 660 to X 640; 2nd, X 609 to X 589 ; 3rd, X 576 to 
X 557 ; 4th (perhaps), X 540 to X 524’5, and possibly another, X 505 to X 484 - 5.* 
The action of zinc and sulphuric acid was tried on these last three solutions, but 
the result obtained showed that no reduction-products similar to those obtained from 
hsematin could be found ; nor, indeed, in the case of vegetable chlorophyll could I 
succeed in getting anything different. Hence the statement that chlorophyll yields 
similar decomposition-products to hsematin is erroneous. 
(8) An orange Uraster with brownish-green coeca.—These extracted with absolute 
alcohol formed a yellow solution, with a red fluorescence, the bands of which read as 
follows : 1st, X 667 to X 649 ; 2nd, X 615*5 to X 593 ; 3rd, X 544 to X 532, and 4th, 
about X 516 to X 488. 
A second extraction of the coeca of the last three specimens furnished the same 
results on examination, the solutions in absolute alcohol being greenish, with a red 
fluorescence, the bands reading: 1st, X 667 to X 649 ; 2nd, X 611 to X 593; 3rd, 
X 543 to X-529'5, and 4th, X 516 to X 488 (?). 
A third extract gave the same results ; hence it is evident that the only pigment 
which could be exHacted by absolute alcohol was enterochlorophyll. 
(9) A brown Uraster yielded similar results, the bands of the solutions of the radial 
coeca corresponding with the above. 
While these nine Urasters all contained a colouring matter whose bands in alcoholic 
solutions agree closely enough when allowance is made for the difficulty of measuring 
* A second and third extraction of these coeca yielded the same spectra as the above, and these 
extracts were used for saponifying, as described further on. 
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