252 DR. C. A. MAC MUNN ON ENTEROCHLOROPHYLL, AND ALLIED PIGMENTS. 
cence, and in a deep layer giving spectrum 15, Chart II., of which the bands read : 
1st, X 678 to X 640 ; 2nd, X 627 to X 600 ; 3rd, X 589 to X 566 ; 4th, X 548*5 to X 529*5 ; 
which are practically the same as the bands of a similar solution of plant chlorophyll. 
On evaporating such a solution, and dissolving the residue in absolute alcohol, a similar 
spectrum is obtained, of which the bands read : 1st, X 678 to X 647, and a shading to 
X 640; 2nd, X 625 to X 598 ; 3rd, X 591 to X 569 ; 4th, X 548'5 to X 532 ; and also a 
lipochrome band beginning at X 494\5 and extending to X 468, also probably a second 
which could not be measured. 
That this pigment should be changed by saponification appeared likely, a priori, 
from the action of caustic soda on an alcoholic solution, which was not the same as in 
the case of plant chlorophyll. On adding this reagent two bands appeared in the red 
at first of equal intensity, the first band being the original chlorophyll band, the other 
when it first appears being much less shaded; the latter then gets darker, and all the 
bands finally appear as shown in spectrum 16, Chart II. The action of nitric acid is 
similar to its action on enterochlorophyll and plant chlorophyll, the solution becoming 
bluish eventually, and giving these bands: 1st, X 669 # to X 638; 2nd, X 613 to 
X 591 ; 3rd, X 577'5 to X 558*5 ; 4th, X 543 to X 521*5, and 5th, about X 505 to X 484*5. 
The most noticeable change with acetic acid was the marked intensification of the 4th 
band from the red, but it is not correct to say that a new band appeared with acetic 
acid, it had been already there and became intensified. 
An absolute alcohol solution of this chlorophyll was saponified by Hajvsen’s 
method. The petroleum-ether solution (after saponification) was yellow and gave two 
bands in the violet half of the spectrum, but also two bands in the red in a deep 
layer. Spectrum 17, Chart II., is the result of combining a deep and shallow layer. 
The presence of the bands in red may be due to the saponifying not having been 
thoroughly carried out. f The other bands read : 1st, X 490‘5 to X 468 ; 2nd, X 458'5 
to X 445. In the second petroleum-ether extract the green constituent was also present 
The yellow constituent could not be made to crystallize distinctly. It became in the 
solid state greenish with iodine in iodide of potassium, a well-marked blue and blue- 
green with sulphuric acid (also a violet and brown tint appeared for a short time), and 
green with nitric acid. Hence it gave the same reactions as the yellow constituent of 
plant chlorophyll. 
The ether extraction removed nearly all the green colouring matter from the soap ; 
it was green and did not show a very distinct fluorescence. Its spectrum is shown in 
spectrum 18, Chart II., and its bands read: 1st, X 669 to X 658*5 ; 2nd, X 649 to 
X 636 ; 3rd, X 613 to X 589 ; 4th, X 576 to X 560 ; 5th, X 545*5 to X 529*5 ; and 
6th, about X 513 to X 496*5 (l). After this extraction the soap-lees w T as, as usual, 
agitated with ether containing alcohol, but, contrary to what I found in the case of 
* The shifting of the hand in red not being as well marked as in other cases, 
f Although in this and other cases I followed Hansen’s directions exactly. 
