254 DR. C. A. MAO MUNN ON ENTEROCHLOROPHYLL, AND ALLIED PIGMENTS. 
The alcohol-ether extract of the soap was yellowish-green and did not show a 
marked fluorescence ; it showed a band in red and another evidently due to the 
presence of a lipochrome. On evaporating it down and extracting the residue with 
absolute alcohol a solution was obtained, giving spectrum 5, Chart III., the band in 
red reading A675 toA 657. 
The mother liquid also gave two bands in red, spectrum 6, Chart III., and the other 
faint bands shown in that spectrum. The first read A 669 to A 654, and second A 63S 
to A 623. 
The residue from a petroleum-ether extract did not give a distinct colour change 
with iodine in iodide of potassium, it became green with nitric acid, and a less distinct 
green with sulphuric acid. In other cases the greenish colour with iodine was better 
marked. 
The occurrence of the one-banded lipochrome in the enterochlorophyll of Ostrcea is 
the rule, as other experiments taught, and it is very difficult to separate it from the 
green constituent; still, although a complete separation of the yellow from the green 
constituent cannot be easily accomplished by saponification, it is quite evident that 
the enterochlorophyll of Ostrcea is composed of these constituents, and bears a most 
remarkable resemblance to plant chlorophyll and to that of Spongillct, differing 
slightly in the fact that it is more decomposed by saponifying than either and in a 
different manner.* 
I saponified an alcohol extract of the liver of Helix pomatia by the same method. 
Just as in the case of Ostrcea and Spongilla the green constituent went into the 
petroleum-ether, giving a band in red from A 672 to A 657, also a lipochrome band 
from A 503 to A 482’5, and the other solutions only contained decomposition-products 
of the green constituent mixed with the yellow. This enterochlorophyll was much 
changed by the saponification, and, like that of Ostrcea , contained a one-banded 
lipochrome. 
The remaining experiments on the saponification of enterochlorophyll were carried 
out on that of starfishes and Mytilus, and owing to greater abundance of material 
gave more satisfactory results. Taking an absolute alcohol extract, giving the 
spectrum described under specimens (4) and (5), supra, and saponifying it, and then 
extracting the soap with various solvents, I found that the petroleum-ether only gave 
one lipochrome band between green and blue, and no bands in red. The ether solution, 
however, showed perhaps one band A 490 - 5 to A 470,t and no band in red. In chloro¬ 
form the yellow residue—left on evaporating the ether—formed a deep yellow 
solution showing only one band, A 505 to A 481, and in deeper layer general absorption 
of the violet and blue, thus resembling Kuhne’s rhodophan or xanthophan. On 
* Although there was only one lipochrome band in the above extracts, yet in some cases the fatty 
matter coloured by the chlorophyll, which separates out on evaporating an alcohol solution, gave two 
bands belonging to the lipochrome. 
f Perhaps another from about X 456‘5 to A 443'5. 
