DR. C. A. MACMUNN ON ENTEROCHLOROPHYLL, AND ALLIED PIGMENTS. 255 
evaporating the chloroform an orange-coloured residue was left, and on touching this 
with a solution of iodine in iodide of potassium it became perhaps redder, with nitric 
acid bluish and then green, with sulphuric acid a blue, blue-green, and in parts 
brownish colour. 
The ether-alcohol solution was yellowish, showing a band in red in a deep layer, 
and absorption of the blue and violet parts of the spectrum, and now all the other 
bands of the chlorophyll spectrum had disappeared, as shown in spectrum 7, Chart 
III. Hence the enterochlorophyll had become changed by saponifying ; it contained 
a yellow and a green constituent, the former a one-banded lipochrome as in the case 
of Ostrcea. 
A solution (alcohol), giving the spectrum described under (7), from the radial coeca 
of several specimens of Uraster was saponified as before. The petroleum-ether extract 
of the soap showed only one band, spectrum 8, Chart III., from A 488 to A 475, and 
in a chloroform extract of the residue from A 516 to A 484'5. The ether solution also 
showed the same band from A 4 94'5 to A 475, and no other. 
The ether-alcohol solution was yellowish, and showed one band in red and strong 
absorption of the blue and violet (and one lipochrome band'"). 
The mother liquid (after removal of these extracts) gave a double band in red, the 
first A 669 to A 651 ‘5, and second A 636 to A 623, and as the original solution showed 
only one band in red, it is quite evident that the enterochlorophyll had become 
changed by saponifying. 
A solution (alcoholic) from the coeca of (13) and (14) was saponified as before. 
The yellow petroleum-ether extract gave two lipochrome bands, the first from A 498’5 
to A 473'5, and the second A 458‘5 to A 445, and no band in red.f 
The ether solution of the soap was greenish-yellow, and left the soap green ; it also 
contained the lipochrome constituent. The alcohol-ether extract was greenish, and 
on concentration showed a band in red, spectrum 9, Chart III., and as before only one 
band in red is seen, showing how the green constituent had been altered. 
The mother liquid (soap-lees) gave two bands in red as before, the first A 665 to 
A 649, and second A 636 to A 623. 
On evaporating a petroleum-ether solution distinct yellow needles, such as Hansen 
describes, were obtained (see Plate 10, fig. 7), and the residue also gave a slate-blue 
and green with sulphuric acid, a green with nitric acid, and it became slightly reddish 
with iodine in iodide of potassium. The alcohol-ether solution also on evaporation 
left crystals, some of which occurred as minute round green granules, and larger 
spherical crystals showing a fine black cross with crossed Nicols. I have also sketched 
these in the drawing (Plate 10, fig. 8), they are evidently the same as those obtained 
by Hansen from vegetable chlorophyll. I also saponified the enterochlorophyll of 
* Even the hand in red is only seen in a deep layer. The lipochrome band is not shown in the map. 
t It is worthy of notice that complete separation is accomplished in this case, when there is a double 
lipochrome band. 
