262 DR. C. A. MAC MUNN ON ENTEROCHLOROPHYLL, AND ALLIED PIGMENTS. 
Comparison of Enterochlorophyll, Chlorophyll of Grass and Spongilla. 
Paludina. 
Limnseus. 
Trochus Z. 
Trochus C. 
Littor'na. 
Patella. 
Grass. 
Sponailla. 
Band. 
.Rectified 
Rectified 
Absolute 
Rectified 
Absolute 
Absolute 
Absolute 
Absolute 
Spirit. 
Spirit. 
Alcohol. 
Spirit. 
Alcohol. 
Alcoho 1 , 
Alcohol. 
Alcohol. 
A X 
X X 
X X 
X X 
X X 
X X 
X X 
X X 
I. 
678 to 
678 to 
672 to 
678 to 
678 to 
678 to 
679 5 to 
678 to 
656 
651-5 
651-5 
657 
654 
654 
636 
640 
II. 
620 to 
618 to 
618 to 
620-5 to 
623 to 
620-5 to 
627 to 
625 to 
600 
600 
593 (?) 
595 
600 
598 
600 
598 
III. 
0 
0 
0 
0 
0 
0 
593 to 
591 to 
Uncertain 
99 
99 
99 
99 
9 9 
566 
569 
IV. 
552 
547 to 
548"5 to 
545-5 to 
548-5 to 
547 to 
551 to 
548 "5 to 
539 
537 
535 
535 
537 
536 
532 
532 
V 
P 
519 to 
p 
519 
496-5 
521-5 
486-5 to 
494-5 
503 
467 
468 
VI. 
? 
492-5 
473-5 
? 
484-5 
477 (with 
NaHO) 
475 
451 
438 
p 
Remarks. 
On allowing for the difficulty of measuring the edges of feeble bands, the errors of 
refraction caused by varying temperatures of room and difference of solvent, a tolerably 
close agreement is apparent with regard to the bands (I.-IV.) in the red half of the 
spectrum. Those in the violet half differ much, not only between enterochlorophyll 
and grass chlorophyll, but between the latter and Spongilla chlorophyll, which is in 
agreement with the result of saponifying. The third chlorophyll band is generally 
missing from the spectrum of enterochlorophyll, but the position of the other bands 
teaches that it is not a decomposition-product of chlorophyll. Especial stress should 
be laid on the X X of band in red, especially on the wave-length of its redward edge; 
this being the most deeply shaded is the last to disappear on dilution.* 
* Prof. Stokes suggested that by adopting the “ fractional ” method of separation I might have more 
easily arrived at the same results. Accordingly this was done, when it was found that the constituents 
of enterochlorophyll could be partially separated from each other. But the behaviour of entero¬ 
chlorophyll is not the same as that of plant chlorophyll under these conditions, as the green constituent 
goes into the spirit, and the yellow into the bisulphide, which proves that the pigments are dissolved in a 
medium differing from that which holds them in solution in the plant.—(July 24, 1886.) 
