276 DR, C. A. MACMUNN ON" MY0HA3MATIN AND THE HISTOH2EMATINS. 
appears mainly, if not altogether, coloured by haemoglobin, at the same time the second 
oxyliaemoglobin band appeared much too dark, and was therefore probably intensified 
by the presence of another band belonging to a histohsematin 
These spectra are better marked in the snowy owl (Nicteci nivea); in this species 
the cortical and medullary portions of the kidney give a beautifully-marked histo- 
heematin spectrum (spectrum 12, Chart II.). The liver and spleen furnish the same 
spectra seen in other species a feeble band at D, and a darker between D and E. 
The stomach-wall contains oxyhaemoglobin maiuly, and this result is interesting 
when compared with the result of examining the gizzard of other birds. 
The pancreas contains a histohsematin. In the lung oxyhsemoglobin only could be 
detected, and this was the case in all vertebrates examined, although not previously 
mentioned. 
At the suggestion of Professor Moseley I examined the gizzards of several birds, 
and found generally only oxyhsemoglobin ; but in many cases I noticed the darkening 
of its second band as referred to above. 
Other birds, e.g., fowls, swifts, sparrows, and owls were examined, with results the 
same as the above. 
Mammals. 
I place the greatest reliance on the results of the examination of the organs and 
tissues of Mammals, because I have been better able to eliminate the influence of 
haemoglobin in them by injecting the blood-vessels thoroughly with salt solution ; 
and in the quite bloodless organs the histohsematin spectra are well marked.! 
1 do not think it necessary to figure the spectra observed in each species of mammal 
examined, because they are all the same practically for each organ and tissue ; but 
describe as briefly as possible the result of the examination of each species. 
In the hedgehog we find histohaematin spectra in the liver, spleen, kidney, and 
stomach-wall, which are the same as those found in the species already described. 
They may also be present in other organs, but I was satisfied at finding them in 
those referred to. 
The guinea pig presents well-marked spectra belonging to the same class, in the liver 
in which the band between D and E is very dark, probably due to the presence of 
free hsemochromogen; in the spleen, in which treatment with ammonia causes the 
spectrum of hsemochromogen to appear, and in the kidney, where, as in the hedgehog, 
a double band occurs between D and E and a feeble band at I) (spectrum 13, Chart II.). 
In the stomach-wall I only observed the bands of oxyhsemoglobin. 
In the supra renal capside the medulla shows the bands of hsemochromogen well 
* See below. 
t Tlie same method was partially successful in removing the influence of hemoglobin in Fishes, 
Reptiles, and Amphibians. 
