DR. C. A. MAC MUNIS' ON MYOH2EMATIN AND THE HISTOH2EMATINS. 277 
marked, in fact its first band is black. In the cortex the spectrum is mainly that of 
oxyhsemoglobin, while the intermediate zone gives the spectrum of a histohsematin. 
These spectra are figured in spectrum 14 and spectrum 15, Chart II. The significance 
of these spectra will be referred to again. 
In the rat (especially after injection of the blood-vessels with salt solution) well- 
marked histohsematin spectra can be seen. The kidney (spectrum 16, Chart II.) is 
remarkable for the resemblance of its spectrum to that of myoheematin, the liver shows 
a feeble band at D, and a darker one between D and E, the pancreas a similar spectrum 
to that of the cat’s pancreas, the spleen, a spectrum like that of the liver, and the 
stomach-wall a beautifully defined spectrum, closely resembling that of the kidney 
(spectrum 17, Chart II.). In the wall of the intestine similar bands occur, and 
the adrenals show a well-marked hsemocliromogen spectrum, which is not as distinct 
after injection with salt solution as it is when no injection has been used. 
The rabbit is a suitable animal for the demonstration of these spectra. In the 
spleen the spectrum observed was the same as before; the liver was remarkable for its 
hmmochromogen-like spectrum (spectrum 1, Chart III.) ; and this spectrum was got 
from lobules which were quite free from blood. In the kidney, the band between D 
and E was single, and occupied approximately the position of the double band usually 
observed there, and was seen in both cortical and medullary parts ; in other cases 
spectrum 3, Chart III., was seen. The adrenals gave the spectrum of hsemochromogen 
in the medulla, and that of a histohmmatin in the cortex (see spectrum 2, Chart III.). 
In the dog the spectra are similar, the spleen, liver, and kidney, stomach-wall, and 
supra-renal body having been examined. The medulla of the adrenals showed the 
haemochromogen bands, but not as distinctly as in the guinea pig and rabbit. I was 
unable to measure the bands of these spectra with sufficient accuracy for wave-length 
calculation until I had examined the blood-free organs of the cat. 
In the cat one can see the edges of bands by bringing the portions of organ or tissue, 
after thoroughly injecting with salt solution, under examination before the slit of a 
chemical one-prism spectroscope, as they are very distinct. Thus for the stomach- 
wall the following measurements were made (see spectrum 4, Chart III.)—the organs 
being blood-free :— 
1st band (before D) A. 613 to X 593, 
2nd band ... X 569 ,, X 563, 
3rd band ... X 556 „ X 551, 
A similar spectrum is yielded by the pancreas, of which the first band extends 
from :— 
X 613 to X 596-5, 
and the others read :— 
2nd band ... X 569 to X 563, 
3rd band ... X 556 „ X 548'5, 
4th band . . . X532 „X513 (?). 
(Chart III., spectrum 5.) 
