June, 1889. 
president's address. 
137 
208. Ag. asterosporus, Quel. Woods. Sept.-Oct. Tricldey 
Coppice ; New Park, Middleton ; Coleshill Pool; Brad- 
nock’s Hayes ; Sutton Park. No doubt overlooked for 
Ay. rirnosus. 
209. Ag. eutheles, B. and Br. Under fir trees. Sept. Red 
Rock Plantation, Edgbaston (Ay. cacuminatus), With., 
198. The Spring and Crackley Wood, Kenilworth, 
Russell, Rlustr. Corley ; Lady Adams’ Garden, Ansty, 
Adams. 
210. Ag. descissus, Fr., var. auricomus , Batsch. Woods. 
Rare. Roots of filbert trees, Edgbaston, With., 239. 
Kenilworth, Sept., 1849. Russell, Rlustr. Ansty, Adams. 
211. Ag. sindonius, Fr. Shady places. Rare. Oct. Red 
Lane, Kenilworth, Russell, Rlustr. Hopsford, Adams. 
212. Ag. geophyllus Sow. On the ground in woods. Not 
frequent. Aug.-Oct. Oversley Wood, Purt., iii., 636. 
Warwick. Perceval . Crackley Wood and the Dale, 
Kenilworth, Russell, Rlustr. Combe Wood, Adams. 
Coleshill Pool; Tricldey Coppice. 
213. Ag. trechisporus, Berk. In woods, amongst ferns. 
Rare. Oct. Combe Woods, Adams. Alveston Pastures. 
(To be continued.) 
BIRMINGHAM NATURAL HISTORY AND MICRO¬ 
SCOPICAL SOCIETY. 
PRESIDENT’S ADDRESS. 
BY W. B. GROVE, M.A. 
(Continued from page 109.) 
There are several other ways in which the cultivation of 
Bacteria on a solid stratum can be conducted, besides that 
previously described. In the first place, if the number of 
germs in our first plate cultivation is so numerous that the 
colonies soon become confluent with one another, it is easv 
to make a second attenuation by taking a small portion of 
the first and mixing it with a further large quantity of 
liquefied nutrient gelatine. All that is necessary for success 
is to thin out the individuals sufficiently and protect the 
plates of glass from the air. Moreover, if an accidental 
germ from the air should fall upon the plate, it remains and 
grows exactly where it falls, and can easily be recognised as 
a stranger. 
But when we have attained a pure stock, it is easier to 
continue the cultivation in a test tube about six inches long; 
the lower third of this is filled with nutrient gelatine, and the 
