On two species of Heterosporium particularly Heterospovium echinulatum 143 
of H. echinulatum are multiseptate and uni-nuclear. The carnation 
disease is spread by the conidia of H. echinulatum transported by 
means of wind and rain from one leaf to another, or to another plant, 
or fallen spores upon the ground may be transported in like manner to 
new plants. Perithecia-like bodies were found upon overwintered material 
of Dianthus. Owing to their being still in the early stages of develop¬ 
ment it was impossible to decide whether they were perithecia or pycnidia. 
The question whether these bodies had any connection, whatever with H. 
echinulatum was not entered into. 
3. Growths differing both in colour and in form were produced by 
one and the same fungus mycelium upon different nutrient media. These 
differences were noticeable in petri-dish and hanging-drop cultures, and 
existed between the aerial mycelia, the surface mycelia, and the sunken 
mycelia. 
4. The conidia of H. Betae and H echinulatum are produced 
acropetalously by a budding process, firstly from the heads of the 
conidiophores, and secondly from the first formed conidia. In both 
the bent and knoted appearance of the conidiophores is due to the pro¬ 
longations which began from the first head, each head being capable of 
producing one prolongation placed at an angle to the previous one. 
Chains of spores containing three spores in a chain are common in H. 
Betae , and usually three such chains are produced upon one head. In 
the young conidiophores of H. echinulatum a few chains containing two 
spores are sometimes present, but these are seldom seen in the mature 
conidiophore. _ 
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