On the Morphology and Development of Phoma Richardiae n. sp. 
303 
The number of spores in each mass is so great that it is rarely 
possible to see the exact connection between them and the chains of 
conidia. In one or two cases, however, where the mass had become 
somewhat disintegrated, after soaking for a month in Lactophenol it was 
possible to see that the conidia chains, like those in sugar solution, sprang 
from a group of 
spores hooked to¬ 
gether by bridging 
tubes (Fig. 5 , 32). 
Development 
may sometimes be 
watched in a 
moist-chamber 
culture where the 
extruded spores 
happen to spread 
out over the sur¬ 
face, instead of 
remaining in 
heaps. Fig. 5 , 
33—38 represents 
gemmae develo¬ 
ped from spores 
extruded on the 
surface of a plum 
agar moist-cham¬ 
ber culture. The 
identity of these 
conidia with those 
produced on the 
4 ‘luft“ mycelium is 
established by 
their agreement 
in form. They 
germinate readily 
on agar. In order 
to see whether the 
plants arising 
from them were 
in any way diffe¬ 
rent from those 
produced from 
pycnospores a 
number were ob¬ 
tained from an old 
Fig. 5 . /—16 and 20 — 22: “Spore-gemmae 1 * in hanging drops 
of Cane Sugar. — 17 — ig: Brown conidia in the same. — 
23, 24: Germinating spores in the same. — 25 — 30: “Spore 
gemmae“ germinating on plum agar. — 31: Mass of ex¬ 
truded pycnospores, with chains of brown conidia arising 
therefrom (on old plum agar culture). — 32: Single chain of 
brown conidia, showing origin (on old horse dung agar cul¬ 
ture). — 33 — 38: Spore gemmae from spores extruded on 
hanging drop of plum agar. — 39— 43: Brown conidia ger¬ 
minating on plum agar. — 44 , 45: Brown conidia from 
spores direct, and on mycelium respectively. — Fig. 1 — 16, 20 — 30, 
33—37 = 608 A; Fig. / 7 —IQ, 32 , 38 — 45 = 263 /i I Fig. 3/ = 153 / r 
culture and spread out over sterile agar in a PETRi-dish. On germination 
(Fig. 5, JÇ — 43) isolated conidia were selected, examined under a high 
magnification to see that no pycnospores ' were adhering, and then, under 
a low power, picked out with two sterile needles, and brought on to hanging 
drops of agar in moist chambers. The resultant plants presented no fea¬ 
tures distinguishing them from those arising from pycnospores. 
