190 
B. B. Higgins. 
The trichogyne and coiled portion of the carpogonial structure soon 
disintegrate but a part of the coiled basal portion was found in material 
killed December 18 th . 
On January 24 th more of the material was examined when acsoge- 
nous hyphae had aeready formed in the base of the perithecium, but no 
sections showing the origin of these hyphae have been obtained. The 
asci are soon formed, but the spores do not mature until about the first 
of May. In some leaves brought into the laboratory and placed in a moist 
chamber on December 12 th mature asci and asco- 
spores were found the last of January; and in leaves 
brought in January 24 th they had matured after one 
week in the moist chamber. 
The asci are eight spored, cylindrical, crowded, 
without paraphyses, and tend to cling together in a 
cluster when crushed out of the perithecium. The 
spores are two celled, colorless and fusiform. 
Pure Cultures. 
Both pycnospores and ascospores grow readily 
in nutrient agar; and pure cultures from both sources 
have been obtained and studied. 
The germinating pycnospores were transferred 
from agar plates to steamed bean pods. The mycelial 
growth is rather slow and is at first pure white. 
Very soon, however, some of the hyphae next the 
bean pods turn dark and also dark colored pycnidia 
are formed. The mycelium is partly within the tissue 
of the bean pod, and the pycnidia usually begin their 
development within the tissue also. Pycnospores 
similar to those formed on the cherry leaves were 
found in great abundance. Often the whole colony 
is covered by waxy appearing masses of spores 
which have exuded from the pycnidia. 
As the culture becomes older “spermogonia” 
appear bearing rod like “spermatia” indistinguishable 
from those found on the yellowed cherry leaves in 
the fall. Also what appears to be abnormal peri- 
thecia are formed. These are pseudoparenchymatous 
structures which apparently begin their formation in 
a manner similar to the development of perithecia on 
cherry leaves, but no asci were ever found in them. 
The walls of the “spermogonia” were also abnormally 
thick but bore normal appearing „spermatia” in great abundance. 
On steamed cherry leaves the mycelium was almost entirely within 
the leaf tissue. Otherwise the growth was very similar to that on the 
bean pods. 
The ascospores also germinate and grow very readily on nutrient 
agar, so that asci containing germinating ascopores were readily found and 
separated from the contaminations. At first long slender conidia (fig. 13) 
somewhat larger than normal pycnospores were abstricted one after the 
Prumis fiennsy Ivanica 
showing approxima¬ 
tely the grouping of 
perithecia on spots 
infected during previ¬ 
ous summer. (Natural 
size.) 
