8 
Journal of Mycology 
[Vol. 9 
Exp. 5. April 25. 
Exp. 6. April 25. 
Exp. 7. April 27. 
Exp. 8. April 27. 
Exp. 32. May 7. 
Exp. 33. May 7. 
Exp. 44. May 14. 
Exp. 45. May 14. 
Teleutospores sown on Sambucus canadensis. 
— Aecidia May 7. 
Teleutospores sown on Onagra biennis. 
— No infection. 
Teleutospores sown on Impatiens biflora. 
— No infection. 
Teleutospores sown on Urtica gracilis. 
— No infection. 
Teleutospores sown on Sambucus canadensis. 
— Spermogonia May 14; aecidia May 24. 
Teleutospores sown on Onagra biennis. 
— No infection. 
Teleutospores sown on Sambucus canadensis. 
— Spermagonia May 23; aecidia May 26. 
Teleutospores sown on Sambucus canadensis. 
— Spermogonia May 23; aecidia May 26. 
It will be seen from the above that, using the teleutospores of 
Puccinia atkinsoniana, successful inoculation followed only when 
the Elder (Sambucus canadensis) was used as the host — Onagra 
biennis, Impatiens biflora, and Urtica gracilis not being suscep¬ 
tible. The Rust was identified by Dr. J. C. Arthur, who in¬ 
formed me that it had been compared with the type. In spite of 
the fact that I had early in the winter entertained the opinion, 
based on field observation, that the alternate form of this Rust 
was the Aecidium on Elder, the result of the first inoculation 
(Exp. i) was a matter of much astonishment, because in the 
meantime it had been learned that aecidia on Elder had been 
obtained, bv Dr. Arthur, by sowings of teleutospores of Puccinia 
bolleyana Sacc. from Carex trichocarpa (cfr. Jour. Mycol. 8:55, 
June 1902). Believing that a mistake might perhaps in some 
way have occurred, additional experiments were instituted in the 
greenhouse, namely, Expts. 2 and 3 above. The result was a 
duplication of the first. The same can be said of Exp. 5, still 
later carried on. I could scarcely maintain a skeptical attitude 
in the case longer, yet it was determined to perform the work 
once again. This time it was decided to select host-plants grow¬ 
ing out of doors in a neighboring grove. The decisive results 
again obtained, see Expts. 44 and 45, could allow no possible 
doubt — especially since the numerous unprotected plants, adja¬ 
cent as well as at a distance, were entirely free from aecidia. 
Finally it may be said that the aecidia obtained by the inoculations 
were apparently the form ordinarily designated as Aecidium 
sambuci Schw. 
PUCCINIA BOLLEYANA SACC.( 4 ) FROM CAREX TRICHOCARPA. 
Exp. 4. April 24. Teleutospores sown on Sambucus canadensis. 
— Few spermogonia May 1; few aecidia May 14. 
Exp. 46. May 14. Teleutospores sown on Sambucus canadensis. 
_ —Few spermogonia May 23; few aecidia May 26. 
( 4 ) Puccinia sambuci (Schw.) Arthur n.n. Bot. Gaz. 35:15. Tan. 
1903. 
