108 
Journal of Mycology 
[Vol. 9 
in contact with them to induce natural infection (Expt. No. 79). 
It was presumed that the spores adjacent to the growing shoots 
might germinate and infect the host. Such proved to be the 
case. Spermogonia appeared at the base of the shoots in two of 
the pots March 23. A week later the characteristic secidia ap¬ 
peared. 
Artificial infection in the usual way was also practiced. The 
teleutospores exposed all winter on stems of Ruellia strepens were 
sown on plants in the greenhouse March 25 (Expt. No. 81). 
Spermogonia appeared April 2, and secidia followed in abundance 
April 10. These were mainly on the upper leaves on which the 
sowings were made. 
^Ecidiospores obtained in Experiments 79 and 81 were used 
to inoculate plants of Ruellia strepens reserved for this purpose. 
This experiment (No. 138) was successful, in the usual time the 
uredospores and teleutospores appearing in great abundance. No 
plants besides those used in the above experiments, though ad¬ 
jacent, exhibited any of the stages of the Rust. From all of the 
foregoing it is experimentally demonstrated that this species is 
an Aut-eu-puccinia. 
With the assistance of Mr. J. G. Sanders and Miss Clara G. 
Mark I am able to illustrate this species; see Plate II. A few 
notes are also added. 
The pycnidia and aecidia on the stems cause usually a slight 
distortion, the tissues of the affected part of the host becoming 
slightly hypertrophied. Fig. 1 shows such a case; this was 
sketched from an artificially infected plant. The distortion in 
consequence of slight hypertrophy in leaves is much less except 
when the midrib is attacked—in which case the curvature may be 
greater than that of the stems. The affected areas of the leaves 
are usually more restricted; the spots are very irregular, mostly 
angular, usually 3-6 mm. in diameter, the border a greenish yel¬ 
low. Those of the stem, petiole and midrib are mostly longer 
6-15 mm.). The sori are a dirty red-brown color. 
The aecidiospores are quite irregular in shape as shown in figure 
4. They have thick walls, verrucose on the surface, and are 
reddish or yellowish-brown in color. A number of our recorded 
measurements are as follows: 33x15, 26x20, 25x18, 30x19, 24x18, 
31x18, 25x20, 35x15, 36x13, 30x22, 30x20/*. 
The sori of the uredo and teleuto stages on the leaves are 
mostly circular or slightly irregular, -J-i mm. diameter. They 
fuse in many cases to considerable extended areas. The sori on 
the stems are much larger. 
The uredospores are brown, echinulate, slightly smaller than 
the secidiospores, as these measurements show: 21x18, 21x16, 
24 Xl 8 , 24 Xl 6 , 30 X 17 , 25 X 15 , 18 X 18 , 25 Xl 8 , 20 X 16 , 24 X 17 (JL 
Numerous spores are shown in fig. 5. 
