Dec. 1903] Uredineous Infection Experiments i?i 1903 
227 
PUCCINIA CAULICOLA Tr. & Gall, from SALVIA LANCE- 
OLATA Muhl. 
Teleutosporic material of this species was kindly sent me 
by Mr. E. Bartholomew in April 1903 from Rockport, Rooks Co., 
Kansas. Cultures were made on seedling plants of Salvia lance- 
olata Muhl. grown in the greenhouse — the seed also furnished 
by Mr. Bartholomew. Some adventive plants of this western 
species had previously been found by a roadside in the suburbs 
of Columbus, Ohio, and they also furnished opportunity for at¬ 
tempted inoculations with the species under consideration. In¬ 
fections were obtained in case of the seedling plants in the green- . 
house — the aecidia appearing in due time though by no means 
abundant. Re-inoculation at a later date, with teleutospores 
from same source, was also successful. 
This demonstrates the existence of an aecidial stage for this 
species — not before reported — and establishes the connection 
of teleutospores and aecidia on the same host. Mr. Bartholomew 
/searched for aecidia in Kansas but found only uredo and teleuto¬ 
spores on the indigenous Salvia lanceolata. Attempted inocula¬ 
tion of the adventive plants at Columbus, Ohio, done under very 
unfavorable conditions, were not successful. 
No spermogonia were seen. A satisfactory diagnosis of the 
aecidia can not be given from the scant material obtained — 
about a dozen sori — but the following may be offered: 
Aecidium caulicolum. — Sori mostly epiphyllous, few, 
scattered, forming sordid-yellowish spots and which are incon¬ 
spicuous on the under side of the leaf, less than 0.5 to 1 mm. or 
more in diameter, circular in outline. On one leaf occurred be¬ 
sides several epiphyllous sori, also a single hypophyllous linear 
sorus, about 6 mm. long, following a prominent vein near the 
base of the lamina which it distorted to semicircular outline. 
Aecidia sometimes limited to one or very few in a sorus, sometimes 
10 or 12 (over two dozen in the single linear sorus above de¬ 
scribed), 210-350 p diameter, when ruptured the edge somewhat 
regularly lacerate but scarcely recurved. Peridial cells very 
large. Aecidiospores pale golden yellow, echinulate, globular 
to oblong or sub-ovate, 10-25 x 17-20 p ; pores not seen. 
Exp. 181. May 28. Teleutospores of Puccinia caulicola Tr. & Gall. 
applied to seedling plants of Salvia lanceolata Willd. June 9 , 
scattered amphigenous aecidia. 
Exp. 242. June 17. Same to Salvia lanceolata Willd. Aecidia noticed 
July 11 , (perhaps appeared earlier). 
Exp. 243. June 17. Same to Urtica gracilis Ait. No infection. 
Exp. 244. June 17. Same to Monarda fistulosa L. No infection. 
Exp. 245. June 17. Same to Salvia lanceolata Willd., adventive plants 
on Greenlawn Ave., Columbus, Ohio. No infection detected. 
