48 
/ 
and did not taper towards the ends, neither were they connected with 
fruiting pustules, but were borne directly on the vegetative mycelium. 
In fact, they seemed to be short, brown, aerial branches which had 
grown out into colorless hyphse. In all the cultures wherever a 
pustule was produced the setra were present, and although none of them 
were made from single spores, there is every reason to believe that 
they were pure cultures of the spores. No setm could be discovered 
among them when carefully examined with the microscope, and they 
are so large as to be easily visible, moreover the seUe are not easily 
detached from the mycelium or pseudo parenchyma at the base of the 
pustule, and in some cases the spores were merely floated off from the 
pustule, so that the black setm could scarcely have been carried with 
them. Besides, as had been said, a microscopical examination of the 
cultures revealed only the spores present. The material did not give 
positive evidence that the setm and basidia sprang from the same hy- 
phae, but some of the very young pustules made this almost certain. In 
case of a similar fungus on cotton, I have seen the setae bearing sports 
similar to those borne on the basidia, but nothing of the kind could be 
seen in this case. 
The time of reproduction in artificial cultures agrees exactly with 
that in nature. Sowing the spores on the leaves of healthy hollyhocks 
in a drop of water produced well developed pustules in seven days. 
Owing to the similarity of this fungus to C. Lindemuthianum an at¬ 
tempt was made to produce it on bean x>ods; this was unsuccessful, but 
inoculations similarly made with spores of G. Lindemuthianum produced 
the spores of that fungus. The inoculations were made by putting the 
spores in incisions made with a flamed knife, attempts to produce the 
bean fungus by sowing the spores on the outside having failed informer 
experiments. 
No trouble was experienced in producing the hollyhock disease on 
healthy plants. For the first experiment three perfectly healthy seed¬ 
lings, growing in a shallow pot in one of the Department greenhouses, 
were selected. There were sixteen plants in the dish and they were so 
close together that their leaves were in contact. The bases of the plants 
where the young leaf was not yet unfolded, and the points of union of the 
blade and petiole of full grown leaves were chosen as points of infection. 
In a week each of these three plants were diseased at one or more of the 
inoculated spots, while the other plants in the same dish were perfectly 
healthy except for a few spots of Cercospora on some of the leaves. 
These spots were entirely distinct from those caused by the Colletotrich- 
ium spores, and there was no possibility of confounding the two fungi. 
Later, two of these infected plants were killed by the fungus passing 
down from the young leaf to the base of the plant. This experiment 
was repeated by inoculating other plants in the same dish and was 
successful each time. The fungus which developed on these plants 
agreed in every particular with the one in Henderson’s greenhouses. 
