150 
In tubes of agar a becco tbe culture is indentical with the preceding, the margin 
. is less bilobed. 
The culture by needle in geiatiue presents a uniform, transparent, finely pointed 
appearance. On the surface of the meniscus the form is irregularly rounded with a 
finely lobed margin as in the preceding. 
In the different materials taken from the olives of Puglia, Calabria, the Yesuvian 
region, and the Sorrentine peninsula, I have demonstrated in each case the same 
microorganism in the cultures. 
In tumors which had been gathered about a year the Bacillus had been destroyed. 
In the cortical tubercles and in their miliary form I have demonstrated the same 
Bacillus. I have performed three series of inoculation experiments. I have practiced 
the same method of inoculation which I had before adopted. 
Series I. Inoculatioil of pure cultures in olive plants. —The plants used were all grown 
from seed, some were raised by myself, others were given me by Signor R. Pecori, of 
Florence, from his establishment. The plants were taken from seed and not from 
cuttings, to avoid heredity from the mother plant which might be infected. 
The inoculations were made April 27 of the current year. By the 1st of June the 
tumors were already evident, and by tbe 1st of July were much developed. The 
controls have not given signs of tumors. These results are the confirmation of 
those obtained roughly by me and with impure cultures in 1887. I am able to con¬ 
clude that the disease of the tuberculosis of the olive (commonly scab) may be produced 
by a specific pathogenic Bacillus which I name Bacillus olece-tuberculosis, understanding 
the tubercle in the sense of botanical pathology. 
Series II. Inoculations of the Bacillus in other plants. —The conditions of inocula¬ 
tion are identical with the preceding and on the same day in the following plants: 
peach, plum, apricot, grape, fig, pear, apple, bitter orange, lemou, rose, Abies excelsa, 
A. pectinata, Cedrus Libani, Till now (July JO) I do not see the least sign of a tuber¬ 
cle; the wounds are perfectly closed and healed. I am able to conclude from this 
that these bacilli are not able to produce the same pathological effects in the plants indicated. 
Series III. Inoculations of other microorganisms in olive plants. —With the identical 
conditions preceding I inoculated into olive plants the following microorganisms 
which I am studying in the said Zoological Station : (1) A bacillus obtained in small 
tubercular swellings of the plum ; (2) a second bacillus obtained as the preceding ; 
(3) a bacillus found in the gums of citrous plants ; (4) one of the bacilli of the pus 
of the citrous plants ; (5) a bacillus of the cancer of the vine. Not one of the many 
inoculations has produced a tumor. Could this be done the tuberculosis might be 
produced by any microorganism whatever. This third series of experiments indicates 
much more certainly the pathogenic power of the Bacillus of the tuberculosis of the olive. 
General observations .—The tubercle of the olive is an excrescence upon 
the limb of the tree which might pardonably be at first mistaken for 
an insect gall. These excrescences or tumors are quite variable in size, 
probably most of them are mature before reaching an inch in diameter, 
but some become large coarse knots. Many branches cease to grow, in 
whole or in part, beyond the tubercle, after the latter has become par¬ 
tially developed. Some branches become stunted while others die 
entirely toward the end. Hence the growth of the tubercle is largely 
limited by the vigor and life of the limb bearing it. 
Dr. Savastano says * that the tubercles occur upon branches from 1 
to 15 years of age. In forming, the tubercle commonly takes its origin 
quite near the cambium zone, though more frequently the center of 
bacteria begins to form in the liber portions of the fibro vascular bun¬ 
dles. To the unaided eye the forming center appears like a very small 
* Comptes Bendus. Paris. T. cm, p. 1144. 
