54 Journal of Mycology [Vol. 11 
species of Helianthns were employed, on which to sow the 
telentospores. 
CULTURES OF HELIANTHUS RUST. 
Hosts for cultures 
1. H. annuus . 
2 . H. decapetalus . 
3. H. divaricatus . 
4. H. grosse-serratus . .. 
5. H. hirsutus . 
6 . H. Kellermani . 
7. H. laetiflorus . 
8 . H. Maximiliani . 
9. H. mollis . 
10. H. occidentalis . 
11. H. orgyalis . 
12 . H. scaberrimus . 
13. H. strumosus . 
14. H. tomentosus . 
15. H. tuberosus . 
! Abundant infection. 
; Infection, but slow growth and few cr no aecidia formed. 
. No infection. 
- Not sown. 
The results of this year accord closely with those of 1902 
and 1903. In 1902 some success was attained in sowing spores 
from H. grosse-serratus on H. Maximiliani , but no success this 
year. In 1903 sowing spores from H. mollis on H. strumosus 
gave no infection, this year a few spermogonia were formed, but 
no further development took place. In all other respects the 
work of the three years is in perfect accord. Altogether sixty- 
four sowings have been made. 
Looking over the accompanying table it will be seen that 
each set of spores grew upon the species of host from which 
derived, but not upon the other two species, except that spores 
from H. laetiflorus sown on H. mollis gave a tardy showing of 
spermogonia, without further development. Also each set of 
spores grew luxuriantly upon H. annuus , and each made a feeble 
growth upon H. tomentosus, but on all other species they either 
failed to infect or made a feeble growth, with the single excep¬ 
tion that spores from H. laetiflorus grew well on H. scaberrimus. 
From all results so far available, it seems that the following 
conclusion may safely be drawn. Puccinia Hclianthi Schw. is 
a single species, having many races, for which H. annuus acts 
as a bridging host. 
4. Puccinia subnitens Diet.— Teleutosporic material of 
this species on Distichlis spicata (L.) Greene was sent from 
Nebraska by Rev. J. M. Bates, to whom I am also indebted for 
suggestions which led to one of the most interesting series of 
cultures yet made. In a communication dated April 30, 1904, 
Source of teleutosporic material 
H. mollis H. grosse-serratus H. laetiflorus 
l t ! 
