March 1905] Cultures of Uredineae in 1904 
55 
Rev. Bates says: ‘‘Last spring I found Aicidium on Cleome 
serrulata in two nearby localities, and on Chenopodium album, 
closely associated with the rust on Distichlis spicata, and no 
other. Now I have it again under same conditions. I wrote 
Mr. Holway about it and shocked him; he does not think it 
possible. But I am well convinced that it is the same as on 
Chenopodium” [proved by cultures in 1902 from Distichlis-rust 
sent by Rev. Bates]. Further reasons were given for believing 
that the Cleome and Chenopodium aecidia came from teleuto- 
spores on Distichlis, among them being that “last year I watched 
for the uredo from it on the Distichlis, and got it May 21, after 
collecting the first Cleome aecidia May 5.” All these observations 
seemed very convincing, yet the deductions seemed very improb¬ 
able. He followed up the statements by sending seedling plants 
of Cleome serrulata and Chenopodium album on which the large, 
brilliant orange-colored aecidia occurred in remarkable profusion, 
and so much alike that in appearance and structure they had to 
be pronounced identical. As the season progressed Rev. Bates 
also sent freshly collected specimens of aecidia on Roripa sinu- 
ata (Nutt.) Hitch., Sophia incisa (Engelm.) Greene, and Salsola 
Tragus L., which he considered belonged to the same inclusive 
species. The results of my cultures confirm the above clever 
observations, and add still other hosts for the aecidium. The 
data are as follows: 
May 17, Sown on Chenopodium album; May 25, spermogonia; June 5, 
aecidia. 
May 17, Sown on Cleome spinosa; May 25, spermogonia; May 30, aecidia. 
May 17, Sown on Lepidium apetalum; May 25, spermogonia; June 7, 
aecidia. 
May 30, Sown on Lepidium Virginicum; June 7, spermogonia; June 16, 
aecidia. 
May 30, Sown on Sophia incisa; June 7, spermogonia; plant injured. 
May 30, Sown on Erysimum asperum; June 5, spermogonia; June 14, 
aecidia. 
May 30, Sown on Cleome spinosa; June 5, spermogonia; June 14, aecidia. 
We have here a demonstration of the remarkable fact, not 
known for any other species of rust so far as I recall, that 
Puccinia subnitens with teleutospores on Distichlis spicata has 
aecidia growing with equal vigor upon species belonging to three 
families of plants. The teleutospores for the above sowings 
were all taken from the same collection, and from only a few 
leaves of the grass, which precludes the possibility of a differenti¬ 
ation in the teleutoform, or any division into races. The aecidium 
is unusually well marked. Going over my herbarium and taking 
out the forms which are identical with this species, beyond a 
doubt, and adding to those already mentioned, we have the fol¬ 
lowing hosts and distribution for the aecidium of this species. 
