March 1905 ] Cultures of Uredineae in 1904 59 
dissimilar to the bright orange-yellow aecidia on Ribes in the 
fields. 
Teleutosporic material was sent by Dr. J. J. Davis from 
Racine, Wis., on Car ex gracillima, which was sown on Ribes 
doridum (3), R. rubrum, and R. aureum (2), with no infec¬ 
tion, except that in one case a few spermogonia appeared on 
R. aureum after eleven days, but reached no further develop¬ 
ment. It was also sown with abundant success on R. Uva-crispa 
(2), R. Cynosbati and R. rotundifolium (2), thus confirming 
previous work. 22 
Excellent teleutosporic material was found on Car ex crinita 
Lam., Lafayette, Ind., and sown May 7, on Ribes rubrum, 
with no infection, on R. Uva-crispa, with weak infection, the 
plant being in poor condition, and on R. rotundifolium with 
strong infection, showing spermogonia May 17, and aecidia 
May 25. Another equally strong infection was secured with 
a sowing on the last host June 3. This culture adds another 
teleutosporic host. 
16. Puccinia Polygoni-amphibii Pers. In previous 
years 23 this rust was sown upon three species of Polygonum and 
Cicuta maculata with no infection. After learning of Dr. Tranz- 
schel’s successful cultures on two European species of wild 
geranium 24 considerable effort was made to secure teleuto¬ 
sporic material with which to repeat the work. No rust could 
be found about Lafayette where it usually occurred. At Spirit 
Lake, Iowa, a diligent search where it had previously been 
common, only gave a few slightly rusted leaves. No better suc¬ 
cess was met with at Fair Oaks, Ind. An appeal to my excel¬ 
lent coadjutors, Prof. W. A. Kellerman, Columbus, Ohio, Rev- 
J. M. Bates, Red Cloud, Neb., and Dr. J. J. Davis, Racine, 
Wis., brought out the same experience, and only added a few 
slightly rusted leaves from Wisconsin. The three meager col¬ 
lections were, however, useless, as none of the teleutospores 
would germinate. I then waited for aecidia to appear on ger¬ 
anium and found them as rare as the teleutospores had been. 
Finally I obtained a few belated aecidia on Geranium macula- 
tum L. ( AE . sanguinolentum Lindl.) and sowed them on 
Polygonum emersum (Michx.) Britt., June 11, and on June 
19, uredospores appeared, and eventually on Aug. 17, teleuto¬ 
spores began to form, the plants being kept in the greenhouse 
during the culture. The American and European forms are 
therefore identical. 
22 Jour. Mycol. 8:53. 1902, and 10:11. 1904. 
23 Bot. Gaz. 35:12. 1903, and Jour. Mycol. 10:9. 1904. 
24 Centr. f. Bakt. 11 2 :106. 1903. 
