July 1905] Notes o?i Seme North American Phyllachoras 
159 
NOTES ON SOME NORTH AMERICAN PHYL¬ 
LACHORAS. 
JOSEPH F. CLEVENGER. 
During the past year I have critically examined a large num¬ 
ber of specimens of several of the species of Phyllachora. At the 
suggestion of the Editor of the Journal, I present here some of 
the more interesting notes made. The material used, most of it 
previously identified, belongs to the herbarium of the Ohio State 
University and to Professor Kellerman’s private herbarium. All 
these and some additional specimens furnished by several botan¬ 
ists were kindly placed at my disposal. Much of the material ap¬ 
parently in good condition proved to be sterile or immature and 
consequently not so much was accomplished in the study of the 
genus as was anticipated in the beginning. 
The genus Phyllachora belongs to the large family Dothide- 
aceae, a group of the higher fungi. There are according to Lin- 
dau in Pflanzenfamilien 200 species of Phyllachora reported from 
the world, of which forty-four have been reported from North 
America and islands adjacent. Of these forty-four I have been 
able to examine material representing twenty-two species, though 
not all in condition favorable for most satisfactory study. 
Plate 78 illunstrates in a general way the different types 
which have been described as Phyllachora, none of which seem to 
have been figured with the possible exception of Figs. 14-16. 
It is to be noted that some species which have been described 
as Phyllachora have only conidiospores, as is illustrated by Fig. 
13. Some show asci with only four ascospores, as is illustrated 
by Fig. 19. Others show asci with sixteen ascospores, see Fig. 
30. In a few cases not heretofore reported I have found asci and 
ascospores. 
This is only a preliminary report on the species of Phylla¬ 
chora and it is intended to carry the work on in the future. Speci¬ 
mens, either determined or undetermined, are kindly solicited and 
may be sent to the Botanical Department of the Ohio State Uni¬ 
versity, Columbus, Ohio. 
The material examined was of course on dry leaves and stems 
of various plants and was prepared for examination by two meth¬ 
ods, namely, imbedding in paraffine, and free hand sections. The 
imbedding was unlike the ordinary in that the material was soft¬ 
ened in warm water from one to three hours and then left in a 
10 per cent, solution of hydrofluoric acid from two to four days, 
treated with agar-agar, and finally imbedded in paraffine. The 
sections were stained on the slides in the ordinary way. The 
iron-alum haematoxylin stain gave the best results for general 
study. This method was most satisfactory in the study of the 
