160 
Journal of Mycology 
[Vol. 11 
perithecia and of the structure of the stroma. In the study of 
the asci and ascospores the best results were obtained by selecting 
a mature stroma, teasing it apart on the slide and staining it with 
eosin. 
NOTES 6N SPECIES. 
Phyllachora trifolii (Pers.) Feld. Fig. 1-4. — This is 
found on several species of Trifolium. On Trifolium wormski- 
oldii collected by E. B. Copeland at Montana Pt., California, 
were found mature asci and ascospores. The asci are cylin¬ 
drical, 8-spores, (Fig. 3), ascospores uniseriate, oval, hyaline, 
8-10 X 5-6/t (Fig. 4). 
Cooke, Grev. XIII: 63, states that the spores are 20 x 10 /a. 
Persoon, Synoposis, 30, did not report finding asci or ascospores. 
Neither did Fuckel in Symbolae Mycologicae report any asci or 
ascospores. 
Further investigation may verify the great difference which 
seems to occur in the above descriptions. Since the descriptions 
coincide in the main, I believe the plants referred to are one and 
the same thing. 
Phyllachora ambrosiae (B. & C.) Sacc. Fig. 5-10.— 
This is found on several species of Ambrosia but generally imma¬ 
ture or sterile. On Ambrosia psilostachya, collected by W. A. 
Kellerman at Manhattan, Kansas, Aug. 1889, asci were found con¬ 
taining eight ascospores. Ascospores uniseriate, elliptical, hyaline, 
17x9/4. 
Cooke, in Grev. 4: 105, on Ambrosia artemisifolia and Am¬ 
brosia elatior, reports practically the same thing, only he says 
nothing about the number of ascospores in an ascus nor the size 
of the ascospores. 
Phyllachora diplocarpa E. & E. Fig. 11-13. — This was 
named and described by J. B. Ellis and B. M. Everhart. At first 
they called it Homostegia diplocarpa, later because of the char¬ 
acter of the stroma they changed it to Phyllachora diplocarpa, 
Bull. Torr. Bot. Club, 24: 135 and 292, 1897. They described it 
from material (on Distichlis maritima) collected by E. Bartholo¬ 
mew in Rooks Co., Kansas, Sept., 1895. They report finding two 
kinds of conidiospores, suballantoid, hyaline, 5-7XI-1J/A; cylin¬ 
drical nucleated spores, becoming 3-septate, 14-23 x 4-4J /a. 
In the same material sent to me by E. Bartholomew only the 
cylindrical 3-septate conidiospores were found. 
This is one of the species of Phyllachora in which only co¬ 
nidiospores have been found. 
Phyllachora graminis (Pers.) Feld. Fig. 14-16. — The 
speciment in question was found on Bouteloua oligostachya which 
was collected by E. Bartholomew at Stockton, Kansas, Oct. 1892. 
