Jan. 1904] 
Cultures of Uredineae in 1903. 
9- 
borne this year in part by the Indiana Experiment Station and in 
part by a grant from the Botanical Society of America. By 
this means I was enabled to have the services of Mr. J. Clyde 
Marquis, an undergraduate student of the university, who made 
part of the preliminary drop cultures and attended to the micro¬ 
scopical technique, and of Mr. Fred J.Seaver, a graduate of Morn- 
ingside College, Sioux City, Iowa, and a fellow in botany at 
the University of Iowa, who made most of the sowings and drop 
cultures and kept the records. The most active period for this, 
work extends from the middle of April to the middle of June, 
while a smaller portion of the work extends through the re¬ 
maining months of the year. The grant from the Botanical So¬ 
ciety also permitted systematic field observations at Fair Oaks 
in the oak barrens of northern Indiana, where many species of 
rusts abound, for the most part unlike those occurring at Lafa¬ 
yette where the chief field observations heretofore necessarily 
have been made. These excursions into an unworked locality 
resulted in the discovery of the Andropogon-Comandra combina¬ 
tion, the undescribed Carex-Solidago combination, and the au- 
toecious character of the wide-spread Lespedeza rust, as well as 
minor items. 
During the present season 68 collections of material were 
employed, and 217 drop cultures were made from them to test 
the germinating condition of the spores. Out of these 26 col¬ 
lections refused to germinate, and were consequently useless. 
There were in all 215 sowings of spores made, representing 32 
species of rusts, and for this purpose were required 72 species 
of hosts temporarily grown in pots in the greenhouse. As in 
previous years success was attained in no case except when def¬ 
inite clues derived from field observations were in hand. 
In order to provide ample resources, as far as possible, so 
that whatever suggestions are obtained even late in the season 
can be tested without delay, a stock of teleutosporic material is 
laid in of any species obtainable. In consequence there are al¬ 
ways some species on hand in germinating condition with no 
definite guide for their use. So far as time permits these are 
sown upon any hosts known to bear aecidia in the region where 
the rust abounds. The results so far have been confined wholly 
to the negative information that the aecidia could not be produced 
on certain hosts. The following is a record of such blind at¬ 
tempts made during 1903. Teleutospores were employed in 
every case. 
1. Uromyces acuminatus Arth. on Spartina cynosuroidcs 
Willd. from Fair Oaks, Ind., was sown on Hydrophyllum ap- 
pendiculatum, with no infection. 
2. Puccinia Polygoni-amphibii Pers. on Polygonum 
emersum (Michx.) Britt, from Columbus, Ohio, and Fair Oaks,. 
